摘要
目的：探索玉屏风散（YPFS）诱导初期给药对Th2（Ⅱ型辅助性T细胞）型接触性超敏反应的效应，并利用HPLC-MS（液质联用）法测定玉屏风散含药血清中的成分。方法：Th2型接触性超敏反应造模采用Balb／c小鼠第1，2天用1．5％的异硫氰酸荧光素（FITC）溶液致敏腹部皮肤，第6天用0．6％的异硫氰酸荧光素（FITC）溶液攻击小鼠耳部皮肤，24h后测定小鼠耳厚和耳重。致敏前给予地塞米松ip剂量为0．67mg·kg-1，YPFS（3．25，6．5g·kg-1，ig），毛蕊异黄酮（0．5，5，10mg·kg-1，ip），芒柄花素（0．5，5，10mg·kg-1，ip）2d，并在致敏第1～3天继续给药3d，第4天停止给药（诱导初期给药）。含药血清检测采用ICR小鼠每天给予YPFS醇提物，连续给药3d，空白对照组给予等体积的生理盐水。最后1次给药后1h取血处死动物。血清经过乙酸乙酯萃取处理后，利用HPLC-MS法进行测定。结果：与正常组相比，模型组小鼠耳肿胀度及耳重显著增加（P〈0．01）。玉屏风散醇提物3．25，6．5g·kg-1诱导初期给药能显著抑制小鼠耳肿胀度（P〈0．01或P〈0．05），减轻过敏性炎症。玉屏风散醇提物含药血清经HPLC-MS测定出3个主要成分，经鉴定分别为升麻素、毛蕊异黄酮、芒柄花素。毛蕊异黄酮和芒柄花素5，10mg·kg-1诱导初期给药能显著抑制Th2型接触性超敏反应模型小鼠耳肿胀度，芒柄花素10mg·kg-1诱导初期给药显著降低耳重。结论：玉屏风散诱导初期给药能显著抑制FITC诱导的小鼠Th2型变应性接触性皮炎的耳肿胀度，含药血清测得的入血成分经过药效学实验验证，对Th2型过敏反应有抑制作用，表明血清中存在抗过敏作用的活性成分。
Objective ： To explore the effect of Yupingfeng san （YPFS） on the initiating stage of allergic inflammatory. Furthermore, YPFS containing serum was detected by HPLC-MS to screen potential active components. Method： The Balb/c mice were topically sensitized with 1.5% fluorescein isothiocyanate （FITC） solution on abdominal skin on the day 1, 2, and elicited on the right ear with 0.5% FITC solution at day 6. Mice were treated with dexamethasone （0.67 mg · kg-1 ）, YPFS （3.25, 6.5 g ·kg-1 , ig）, calycosin （0.5, 5, 10 mg ·kg-1, ip）, formononetin （0.5, 5, 10 mg-kg-1, ip） or normal saline before sensitized for 2 days and then treated with YPFS from day 1 to day 3 （initiation phase）. Ear thickness was measured using a calibrated thickness gauge 24 h after the elicitation. For chemical analysis, ICR mice were administrated with 34. 14 g ·kg-1 YPFS by ig, twice a day for 3 days, saline administrated as control. One hour after the last administration the mice blood were collected via the ophthalmic vein and then detected by HPLC-MS. Result： Compared with the normal group, the ear thickness and weight in model group were significantly higher. The ear thickness and weight were inhibited significantly by administration with YPFS 3.25, 6.5 g ·kg 1 in initiation phase. Cimifugin, claycosin and formononetin were remarkably detected in the YPFS containing serum, while these chemicals were almost undetectable in blank serum. Calycosin and Formononetin 5, 10 mg · kg-1 could significantly inhibit the ear thickness in FITC treated mice, Formononetin 10 mg · kg -1 could increase the ear weight. Conclusion： Administration with YPFS in initiation phase could inhibit Th2 cell-mediated allergic contact dermatitis in mice significantly and cimifugin, claycosin and formononetin detected in YPFS containing serum might be the potential active components with antiallergic action.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第12期145-149,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81073121,81373549)
江苏高校优势学科建设工程
“青蓝工程”项目
国家教育部留学回国人员科研启动基金
南京中医药大学科技创新团队
