摘要
目的:检测CD44基因mRNA在结肠癌组织中的表达。方法:收集25例明确诊断的结肠癌组织及其相应癌旁组织,提取总RNA并逆转录成cDNA。采用Real-time PCR方法检测CD44基因在结肠癌组织和癌旁组织中的mRNA水平。结果:CD44基因在结肠癌组织中的含量明显高于癌旁组织(P<0.05)。CD44基因与患者的性别(P=0.804)、年龄(P=0.592)、分化(P=0.443)、淋巴侵袭(P=0.525)、静脉侵袭(P=0.592)、肿瘤大小(P=0.592)和病理分期(P=0.824)等因素无关。Kaplan-meier生存曲线分析显示CD44基因与结肠癌患者预后具有显著相关性(P=0.041)。结论:CD44基因mRNA在结肠癌组织中表达异常升高,提示CD44可能与结肠癌的发生、发展有密切关系。
Objective：To determine the expression of CD44 gene mRNA in colorectal cancer. Methods：Twenty five colorectal cancer tissues and matched normal tissues were collected by surgery,total RNA extraction and reverse transcription into cDNA. The levels of CD44 mRNA were detected by using Real-time PCR. Results：The levels of CD44 mRNA were higher in cancer tissues than that in normal tissues（P〈0. 05）. High CD44 expression was not as-sociated with sex（P=0. 804）,age（P=0. 592）,differentiation（P=0. 443）,lymphatic invasion（P=0. 525）,venous invasion（P=0. 592）,tumor size（P=0. 592）and pN category（P=0. 824）. CD44 expression was associated with sur-vival rate of the patients with colorectal cancer by using Kaplan-meier survival curve analysis（P=0. 041）. Conclu-sion：Abnormal high CD44 mRNA expression was observed in colorectal cancer tissues,which suggest that there was association between CD44 and the development of colorectal cancer.
出处
《现代肿瘤医学》
CAS
2014年第6期1363-1365,共3页
Journal of Modern Oncology
基金
辽宁省科技厅自然科学基金项目(编号:2013020158)
关键词
结肠癌
CD44
预后
侵袭
病理特征
colorectal cancer
CD44
prognosis
invasion
pathological feature
