摘要
目的 观察表皮生长因子受体 (EGFR)在小鼠胎肺发育过程的表达特征 ,研究表皮生长因子 (EGF)和转化生长因子 α(TGF α)通过与EGFR的作用 ,对胎肺形态发生和肺泡上皮成熟分化的作用。方法 昆明小鼠 ,13- 19天胎肺组织、生后1、7、14、30天及成鼠肺组织共 12组 ,常规石蜡切片 ,采用免疫组化SP两步法检测EGFR的表达。结果 胎肺假腺期 ,EGFR的表达主要定位于支气管呼吸道上皮 ,小管期EGFR阳性反应达到最高峰。在原始肺泡期 ,阳性反应主要在肺泡上皮细胞。出生后 ,支气管上皮细胞EGFR表达重新呈阳性表达。结论 在胎肺发育的不同时期 ,EGFR在上皮细胞的定位有迁移 ,免疫组化反应强弱也有差异 ,说明EGFR在胎肺不同发育阶段发挥不同的功能 ,它不仅参与支气管树的形成 ,还对呼吸道上皮细胞和肺泡上皮细胞的成熟分化有重要调节作用。
Objective To study the distribution of the epidermal growth factor receptor(EGFR) in fetal and postnatal mouse lungs,and to explore the important effects of the Epidermal growth factor(EGF) and Transforming growth factor α(TGF α) which were involved in cell proliferation and epithelial differentiation and maturation through binding to the EGFR. Method Fetal lungs ranging from the 13th to 19th day and postnatal lungs of Kunming mice,totally 12 groups,paraffin sections(5μm),were examined by immunohistochemistry. Results It is showed that EGFR mainly localized in the airway surface epithelium during the pseudoglandular stage,and reached the peaks at canalicular period. In the saccular and alveolar period,its reaction maintained in low level,and at that time,EGFR was shown in alveolar cells. The positive reactions were localized in the airway again in the postnatal lung. Conclusion The presence of EGFR was respectively different at individual developmental stages and in different locations during the lung development. Which is suggested that its functions are changing in different stages,and it not only modulate the process of branching morphogenesis,but also may be involved in epithelial maturation and differentiation.
出处
《解剖学研究》
CAS
2000年第2期107-109,T001,共4页
Anatomy Research
基金
国家自然科学基金资助项目 (NO .3 9770 3 0 0 )
国务院侨办基金资助项目
