摘要
[目的]通过建立帕金森病大鼠模型,观察头穴电针对帕金森病大鼠行为学的影响,同时检测帕金森病大鼠黑质中脑源性神经营养因子(Brain-derived neurotrophic factor,BDNF)及其受体酪氨酸激酶B(Tyrosine kinase-B,TrkB),以及黑质中cAMP反应元件结合蛋白(cAMP responsive element binding protein,CREB)的变化,以探讨针灸治疗帕金森病的可能机理。[方法]用纹状体内多位点注射6-OHDA建立帕金森病大鼠模型,造模成功者随机分为电针治疗组、模型对照组、假手术组和正常组,分别进行头穴电针治疗。疗程结束后采用免疫组化法检测黑质中BDNF、TrkB、CREB水平。[结果]头穴电针治疗组大鼠治疗后较治疗前旋转次数明显减少(P<0.05),模型对照组和电针治疗组黑质内BDNF、TrkB和CREB阳性神经元数量显著低于正常组和假手术组(P<0.01),电针治疗组BDNF、TrkB和CREB阳性神经元数量高于模型对照组(P<0.05)。[结论]头穴电针可明显改善帕金森大鼠的旋转行为,增加黑质中BDNF、TrkB和CREB的阳性细胞数。其机制可能为通过增加黑质中BDNF和TrkB的含量,激活神经元信号保护通路从而使CREB活化,促进了BDNF在黑质内的自分泌,保护DA能神经元。
[Objective] The experiment wil study the effect of the electroacupuncture（EA） on PD rats that of Brain-derived neurotrophic factor（BDNF）, tyrosine kinase-B（TrkB） and cAMP responsive element binding protein（CREB） in order to know the possible mechanisms. [Methods]The research created rats model of PD was caused by injecting 6-hydroxydopamine into the left striatum from 4 points use stereotaxic apparatus. Then histochemical and im-munohistochemical staining methods were adopted to observe the therapy effects of the BDNF, TrkB and CREB content in substantia nigra. [Results]（1） In EA group, the number of rotations after the treatment was lower than before the treatment（P〈0.05）, but that didn＆#39;t change in PD group（P〉0.05）.（2） The number of BDNF, TrkB and CREB positive neurons in PD group was obviously less than those of the normal group and control group（ P〈0.01）.In the EA group, the number of BDNF, TrkB and CREB positive neurons was more than that of the model group（ P〈0.05）.[Conclusion]EA on head can increase the number of BDNF, TrkB and CREB in substantia nigra, thus enhances BDNF in the substantia nigra from the secretion and protects DA neurons.
出处
《浙江中医药大学学报》
CAS
2014年第3期327-332,共6页
Journal of Zhejiang Chinese Medical University
基金
浙江省教育厅资助项目(Y201017904)
国家中医药管理局中医药重点学科(针灸学)(国中医药发20090030号)~~
关键词
帕金森病
电针
脑源性神经营养因子
Parkinson＇s disease
electroacupuncture
brain-derived neurotrophin factor
