摘要
目的探讨骨髓基质干细胞(BMSCs)对lactacystin帕金森病(PD)大鼠的神经保护作用。方法取成年雄性SD大鼠骨髓,培养、分离、增殖和纯化BMSCs,培养至4～6代用于细胞移植。在大鼠单侧黑质致密部(SNc)立体定向注射蛋白酶体抑制剂lactacystin8μg,建立表达Lewy小体并向偏侧旋转的PD大鼠。实验组采用立体定向技术向纹状体区注射BMSCs,对照组注射等体积的生理盐水。观察移植前后大鼠的旋转行为变化以及BMSCs在脑内的脑源性神经营养因子(BDNF)表达、迁移及分化情况。结果实验组中71.43%的大鼠接受BMSCs移植后阿朴吗啡诱导的旋转行为较移植前明显改善(P<0.05),而对照组均未见旋转行为变化。BMSCs在大鼠脑内可以广泛迁移,分布于纹状体、胼胝体和皮质等部位,并表达BDNF;植入的BMSCs未能分化为多巴胺能样神经细胞。结论BMSCs很可能是通过分泌BDNF对lactacystinPD大鼠发挥神经保护作用。
Objective To evaluate the protective role of bone marrow stromal cells （BMSCs） to lactacystin-Parkinson＇s disease （PD） rats. Methods BMSCs were obtained from adult male SD rats and were expanded, isolated and purified. BMSCs were harvested from 4 to 6 passage and used for transplantation. PD rats that expressed Lewy body and presented lateral rotation were made by injecting 8μg lactacystin into one side of substantia nigra compact part of rat with stereotaxic technique. The rats were transplanted with BMSCs labelled by Brdu by stereotaxic unilateral injection into striatum and transplanted with normal saline as control. The behavior changes of rats and the expression of BDNF, migration, differentiation of BMSCs were examined. Results 71.43% of the rats displayed significant improvement of contralateral behavior after BMSCs transplantation, however there was no change in control group. BMSCs were found in several areas of the brain including striatum, corpus callosum and contralateral cortex. The implanted BMSCs expressed BDNF but no tyrosine hydroxylase. Conclusion BMSCs may play a neuroprotective role in lactacystin-PD rats by secreting BDNF.
出处
《临床神经病学杂志》
CAS
北大核心
2006年第4期290-292,共3页
Journal of Clinical Neurology
关键词
帕金森病
骨髓基质细胞
神经营养因子
Parkinson＇s disease
bone marrow stromal cells
neurotrophic factor
