摘要
目的探讨帕金森病(PD)大鼠海马区增殖神经干细胞(NSCs)的分化情况。方法将6羟基多巴(6OHDA)注入大鼠纹状体内制作PD动物模型。连续注射5嗅脱氧尿核苷(Brdu)14d后处死。分别用Brdu和神经核抗原(Neun)以及Brdu和胶质纤维酸性蛋白(GFAP)免疫双标组织化学方法检测增殖的NSCs向神经元和神经胶质细胞的分化情况。结果PD模型成功后7d,在海马区Brdu/GFAP、Brdu/Neun阳性细胞开始出现,14d后双标的阳性细胞数逐渐增加,28d后达高峰。在这些双标的细胞中,Brdu/GFAP阳性的细胞数较多,而Brdu/Neun阳性的细胞数较少。结论6OHDA纹状体内注射制作的PD大鼠模型海马区增殖的NSCs大部分分化为神经胶质细胞,少部分分化为神经元。
Objective To explore the differentiation of neural stem cells （NSCs） in hippocampus of rat model of Parkinson＇s disease （PD）. Methods Rat models of PD were made by injection 6-hydroxydopamine （6-OHDA） into striatum. 5-bromodeoxyuridine （Brdu） was injected into the abdomens of PD rats for 14 days before they were killed. The expressions of Brdu/Glial fibrillary acidic protein （GFAP） and Brdu/Neuronal nuclear antigen （Neun） were determined by immunocytochemistry. The distinctive marker for Brdu/GFAP and Brdu/Neun labeling method was used to determine the phenotype of the differentiated cells. Results After PD models were established, the Brdu/GFAP and Brdu/Neun positive cells appeared at 7 d, increased gradually at 14 d, and peaked at 28 d. In these double labelled cells, Brdu/GFAP positive cells were more than Brdu/Neun positive cells. Conclusions Direct injection of 6-OHDA into the striatum stimulates the proliferation of inherent NSCs in hippocampus of rats. Large amounts of NSCs differentiate into astrecytes and small amounts of them turn into neurons.
出处
《临床神经病学杂志》
CAS
北大核心
2006年第4期293-295,共3页
Journal of Clinical Neurology
关键词
帕金森病
大鼠
神经干细胞
分化
海马区
Parkinson＇s disease
rats
neural stem cells
differentiation
hippocampus
