摘要
建立大鼠2/3肝切除模型,分别于术后恢复0 h、6 h、12 h、24 h、48 h、72 h、120 h、168 h等时间点,采用原位胶原酶消化和密度梯度离心法分离、纯化再生肝细胞,获得的再生肝细胞活性95%以上,纯度96%以上。用DMEM培养液添加其他物质后培养再生肝细胞,在培养的96 h内,肝细胞生长状态良好,具有分泌白蛋白和合成尿素的功能。细胞的DNA合成主要在培养24 h以后,72 h时达到高峰。
Hepatocytes of rat treated by partial hepatectomy after 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 120 h, and 168 h were isolated and purified by collagenase digestion and density gradient centrifugation. The viability of regenerated hepatocytes was over 95% , and the purity was more than 96%. The regenerated hepatocytes grow well in DMEM containing 10% fetal calf serum, and were capable of secreting albumin and synthesizing urea. DNA synthesis of hepatoeytes was initiated after 24 h and achieved the peak at 72 h.
出处
《四川动物》
CSCD
北大核心
2014年第3期440-444,共5页
Sichuan Journal of Zoology
基金
河南省基础研究与前沿项目(122300410253)
