摘要
研究不同温度对斑鳜(Siniperca scherzeri)胚胎发育的影响。结果表明:在18-28℃温度范围内,斑鳜胚胎均能孵化出膜;随温度升高,胚胎发育达囊胚期、原肠期和出膜期的时间均相对缩短,温度对囊胚期前的发育进程影响较小,胚胎发育至囊胚期后,温度升高胚胎发育进程明显加快;孵化时间随温度的升高而缩短,孵化时间T与温度x呈极显著的负相关关系,T=306 738x^-2.463,R^2=0.996 6(P〈0.01);在18-26℃温度范围内,胚胎发育到原肠中期的死亡率较低,孵化率较高,且组间差异不显著(P〈0.05),温度达28℃时,胚胎发育到原肠中期的死亡率极显著上升,孵化率下降极显著(P〈0.01),孵化率y与温度x之间存在极显著的二次曲线相关关系,y=-1.032 1x^2+44.107x-379.99,R^2=0.917 4(P〈0.01)。斑鳜受精卵孵化的最适温度范围为20-26℃。
The effects of temperature on the embryonic development of Siniperca scherzeri were investigated in this study. The results showed that the embryo could be hatched at the range of 18 - 28 ℃,and with the increase of temperature,the periods of embryonic development reaching blastula,gastrula and hatching stage were relatively shorter. Temperature had little effects on the development before blastocyst stage. After embryos developped into blastocyst stage,elevated temperature significantly speed up the process. Incubation time shortened with the increase of temperature. There was a very significant negative correlation between incubation time( T) and temperature( x),and the equation was T = 306738x^- 2. 463, R^2= 0. 9966( P〈0. 01). The mortality rates were lower and the hatching rates were higher when embryonic developped into the mid gastrula stage at the range of 18 - 26 ℃,and the difference between groups was not significant( P〈0. 05). When the temperature reached 28 ℃,the mortality rate of the mid gastrula stage increased very significantly,and the hatching rate decreased significantly( P〈0. 01). There was a significant square curve correlation between hatching rate( y) and temperature( x),and the equation was y =-1. 0321x^2+ 44. 107x- 379. 99,R^2= 0. 9174( P〈0. 01). The optimal temperature range for the hatching of fertilized eggs was 20 - 26 ℃.
出处
《淡水渔业》
CSCD
北大核心
2014年第3期104-107,共4页
Freshwater Fisheries
基金
福建省海洋与渔业厅重点项目(闽海渔[2010]2-17)
福建省科技厅公益类科研院所专项(2010R1001-1)
福建省科技厅星火计划重点项目(2013S0018)