摘要
以斑马鱼(Danio rerio)为受试动物,研究了四种环境雌激素-17β-雌二醇(E2)和三种增塑剂(DMP、DBP、DOP)对其内脏团的氧化损伤及应激效应。经急性毒性实验,得到E2、DMP、DBP、DOP对成体斑马鱼96 h的半数致死浓度(LC50)分别为2.51、12.33、9.67、9.89 mg·L-1。在此基础上分别设置5个浓度梯度,研究E2(暴露2 d,4 d)、DMP、DBP、DOP(暴露4 d)对斑马鱼内脏团的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶(GST)活性以及丙二醛(MDA)含量的影响。结果表明,SOD、CAT、GST、MDA对这四种环境激素都非常敏感,其中SOD、CAT、GST活性的影响均呈现先诱导后抑制的趋势,而MDA含量则随着污染物浓度的升高而增加。高浓度暴露条件下,E2(0.4 mg·L-1)和DMP、DBP、DOP(0.8 mg·L-1)对SOD、CAT、GST活性均有显著抑制(p<0.05),MDA的含量有显著升高(p<0.05)。可见,E2、DMP、DBP、DOP会导致斑马鱼内脏团氧化损伤,并且在同等浓度下E2的毒性明显高于三种增塑剂。
The oxidative stress effects and damage of four kinds of environmental estrogens （E2, DMP, DBP, DOP） on viscer- al mass of Danio rerio were investigated. Through the acute toxicity test, the median lethal concentrations （LC50） of E2, DMP, DBP, DOP to the Danio rerio for 96 h were obtained, which were 2. 51,12. 33, 9. 67, 9. 89 mg. L^-1 , respectively. Then five gra- dient substrate concentrations were set up respectively based on the LC50s and the effects of E2 （exposure 2 d and 4 d） , and DMP, DBP, DOP（ exposure 4 d） on SOD, CAT, GST activities and MDA content in visceral mass of Danio rerio were studied. The results showed that SOD, CAT GST and MDA were sensitive to these four kinds of environmental estrogens. The activities of SOD, CAT and GST were induced flint and then inhibited, and MDA content increased with the rising of substrate concentration. The activities of SOD, CAT and GST were inhibited and MDA content increased significantly under high substrate concentration （0. 4 mg.L^ -1 for E2 and 0. 8 mg . L^-1 for DMP, DBP, DOP）. Therefore, E2, DMP, DBP, and DOP can lead to oxidative damage in visceral mass of Danio rerio and E2 is markedly higher than the other three plasticizers in toxicity under the same concentration.
出处
《环境科学与管理》
CAS
2014年第4期30-35,共6页
Environmental Science and Management
基金
国家自然科学基金资助项目(51178046)
环保公益性行业科研专项资金资助(201109041
201209048)
