摘要
建立了固相萃取-气质联用法(SPE-GC-MS)测定水体中9种环境雌激素:雌酮(E1)、17b-雌二醇(E2)、雌三醇(E3)、4-辛基酚(OP)、4-壬基酚(NP)、双酚A(BPA)、己烯雌酚(DES)、美雌醇(EE3ME)和17a-炔雌醇(EE2)的检测方法,优化了萃取和衍生化反应条件,实验结果表明:HLB柱回收率较高,衍生化反应的温度为75℃,衍生时间为90 min;本方法对9种环境雌激素的加标回收率为60.5%～126.7%,对应的相对标准偏差为0.8%～11.1%,方法检出限为1.08～8.60 ng/L,并将本方法应用于实际环境水样的检测。
Nine target compounds including estrone （ E1 ）, 17/3-estradiol （ 17fl-E2 ）, estriol （ E3 ）, 4-oetylphenol （ 4-OP ）, 4-nonylphenol （ 4-NP ）, bisphenol A （ BPA ）, diethylstilbestrol （ DES ）, mestranol （EE3ME） and 17a-ethinyl estradiol （17a-EEl） were simultaneous detected by SPE-GC-MS. Extraction and derivatization conditions were optimized. The results showed that the average recoveries of the nine target estrogens extracted by Oasis HLB cartridges were higher than those by Satara cartridges, the optimal temperature of derivatization reaction was 75 ~C and the optimal time of derivatization reaction was 90 rain. The average recoveries of the nine target estrogens by Oasis HLB cartridges were 60.5% -126.7%, and the relative standard deviations （RSD） were 0.8% -11.1%, and the limits of detection （LOD） ranged from 1.08 ng/L to 8.60 ng/L. This method has been employed to detect the estrogens in environment samples from rivers.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2013年第3期422-426,共5页
Chinese Journal of Analytical Chemistry
基金
湖北省国际合作项目(No.2010BFA019)
湖北省自然科学基金项目(No.2009CDB265)
湖北省农科院青年科学基金项目(No.2012NKYJJ17)资助
关键词
环境雌激素
固相萃取
气相色谱
质谱联用法
River water estrogens
Solid phase extraction
Gas chromatography-mass spectrometry
