摘要
目的:制备羧甲基壳聚糖载药纳米微球,醋甲唑胺为模型药物,测量药物的包封率和纳米微球形态。方法:采用乳化交联法,在微乳液的基础上制备载药纳米微球,对可能影响药物包封率的处方因素进行优化设计,筛选出最优配方。结果:羧甲基壳聚糖溶液的浓度对包封率有显著性影响,三聚磷酸钠溶液浓度和醋甲唑胺药量对包封率未见影响。优化方案的载药纳米微球包封率为49.36%,其电镜下为较规整的球型纳米微球,平均粒径386.0 nm。结论:采用乳化交联法,可形成较高包封率的羧甲基壳聚糖-醋甲唑胺纳米微球。
Objective： To prepare carboxymethyl chitosan （CMC） drug - loaded nanoparticles, with methazolamide as model drug. The drug encapsulation efficiency and morphology should be measured. Methods ： Drug - loaded nanoparticles were preparated in microemulsion by emulsion crosslinking method. The design of formulation factors that may affect the drug entrapment efficiency was optimized to select optimal formulation. Results ： The concentration of carboxymethyl chitosan solution has a significant effect on the entrapment efficiency, when sodium tripolyphosphate （TPP） solution concentration and weight of methazolamide don＇ t effect on the entrapment efficiency. Entrapment efficiency of optimization of drug - Loaded nanoparticles was 49.36%. The nanoparticles is regular and baLL - type, with average particle size in 386.0 nm on electron microscope. Conclusion： Nanoparticles can be formated with hydrophilic carboxymethyl - chitosan coating lipophilic methazolamide by emulsion crosslinking method, and high encapsulation efficiency can be achieved.
出处
《天津药学》
2014年第2期10-13,共4页
Tianjin Pharmacy
基金
天津市卫生局科技基金(青年)项目(No.2012KY34)
关键词
羧甲基壳聚糖
纳米微球
醋甲唑胺
乳化交联
carboxymethyl - chitosan, nanoparticles, methazolamide, emulsion crosslinking
