摘要
目的 :研究结肠腺癌细胞株经不同化疗药物诱导后Fas FasL表达与癌细胞凋亡的关系 ,探索敏感化疗药物的筛选。方法 :将顺铂、丝裂霉素、氟尿嘧啶、表阿霉素按PPC(血浆峰浓度 )、1 10PPC、1 5PPC、5PPC、10PPC加入结肠腺癌LS174T细胞、LoVo细胞共孵 ,用流式细胞术 (FACScan)检测癌细胞Fas、FasL表达率及细胞凋亡率 ,并提取DNA进行琼脂糖凝胶电泳。结果 :FACScan检测显示LS174T细胞顺铂组、丝裂霉素组、表阿霉素组Fas表达率、细胞凋亡率在 1 5PPC或PPC时最高 ,Fas表达率与细胞凋亡率呈正相关 (P <0 .0 5 )。LoVo细胞经化疗药物诱导后Fas表达率与细胞凋亡率无显著相关 (P <0 .0 5 )。结论 :FACScan检测的癌细胞凋亡率可用于敏感化疗药物及其合适剂量的筛选 ,Fas表达率可能仅适用于部分癌症病人化疗药物的筛选 ;
Purpose:To study the relationship between Fas/FasL expression and apoptosis of colon adenocarcinoma cell lines induced by chemotherapeutic drugs and to screen out hypersensitive drugs. Methods:Colon adenocarcinoma cell lines LS174T and LoVo were incubated respectively in cultural media with DDP,MMC,5 FU and EPI at concentrations of PPC(plasma peak concentration),1/10 PPC,1/5 PPC,5 PPC and 10 PPC. Fas/FasL expression and apoptosis were assessed by FACScan, and DNA ladder was examined by agarose gel electrophoresis. Results:In LS174T cells, Fas expression and apoptosis of DDP group, MMC group and EPI group reached their highest peaks at 1/5 PPC or PPC,and Fas expression and apoptosis were positively correlated by relativity analysis. There was no significant correlation between Fas expression and apoptosis in LoVo cells. Conclusions:We could find out hypersensitive drugs and their appropriate dosage according to apoptosis, partly according to Fas expression. The different apoptosis paths between LS174T cells and LoVo cells demonstrate the importance of individualized selection of chemotherapeutic drugs.
出处
《中国癌症杂志》
CAS
CSCD
2001年第1期25-28,共4页
China Oncology
基金
上海市卫生系统百名跨世纪优秀学科带头人资助项目!(No:98RB0 2 4)
