摘要
目的 :探讨中药蟾蜍灵诱导白血病HL6 0细胞凋亡作用。方法 :应用台盼蓝染色法测定HL6 0细胞的生长曲线 ,应用相差显微镜、电子显微镜和DNA琼脂糖凝胶电泳等技术分析细胞凋亡。结果 :蟾蜍灵与HL6 0细胞共育时 ,能明显抑制细胞生长 ,细胞呈典型的凋亡形态学改变 :细胞皱缩 ,染色质凝集 ,沿核膜内侧分布 ,呈新月形 ,可见由膜包被细胞内容物的凋亡小体的产生 ;细胞DNA裂解成 180～ 2 0 0bp及其倍数的片段 ,电泳得到特有的梯形条带。结论 :蟾蜍灵诱导细胞凋亡是其抗肿瘤的作用机制之一。
Objective:To study the mechanism of inhibiting actions of bufalin on human leukemic cells(HL60).Methods:HL60 cells were incubated with bufalin at different concentrations and growth inhibition was analyzed by trypan blue staining. Cell apoptosis was evaluated by phase contrast microscopy, transmission eletron microscopy and agarose gel electrophoresis.Results:Bufalin remarkably inhibited the growth of HL60 cells. Apoptosis of HL60 cells could be effectively induced by bufalin at concentration of 0.01 μmol·L -1 or higher, showing the apoptotic changes in morphology, including cell shrinkage,chromatin agglutination and formation of apoptotic corpuscula; DNA cleavage was observed with agarose gel electrophoresis.Conclusion:Bufalin can effectively induce apoptosis of HL60 cells, which is possibly one of the mechanisms for anti cancer of bufalin. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2001年第1期59-61,共3页
China Journal of Chinese Materia Medica
