摘要
目的 :研究腺相关病毒增强型质粒载体介导的 p5 3基因对血管平滑肌细胞 ( VSMC)的作用 ,探讨其用于防治冠状动脉旁路术后移植血管再狭窄等心血管内外科疾病的可能性。方法 :构建了野生型 p5 3基因的腺相关病毒增强型质粒表达载体 ,通过阳离子脂质体 Dosper介导 ,体外转染VSMC。聚合酶链技术检测外源基因表达。应用细胞计数及 DNA合成分析技术 ,测定 p5 3基因对VSMC增殖的抑制效应。结果 :外源性 p5 3基因可有效导入 VSMC并表达 ;p5 3基因导入可显著抑制细胞生长 ,DNA合成减少。结论 :野生型 p5 3基因腺相关病毒增强型质粒载体转染
Objective:To study the effect of extrinsic wild type p53 gene mediated by adenoassociated virus plasmid vector on vascular smooth muscle cells(SMCs) and the possibility of p53 gene therapy for vessel graft restenosis after coronary artery bypass.Methods:Human wild type p53 gene adeno associated virus plasmid vector was constructed and transfected into rabbit vascular SMCs mediated by the cationic liposome Dosper in vitro.The gene expression was determined by polymerase chain reaction technique.Proliferation of SMCs was investigated by cell counting and isotope incorporation.Results:The extrinsic wild type p53 gene was effectively transferred into the cells with adeno associated virus plasmid vector.The overexpression of p53 gene restricted the proliferation of SMCs and decreased the DNA synthesis.Conclusion:The constructed p53 adeno associated virus plasmid may play a role in gene prevention of vessel graft restenosis after coronary artery bypass.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2001年第1期1-4,共4页
Journal of Zhejiang University(Medical Sciences)
基金
卫生部科学研究基金
国家自然科学基金资助项目
