摘要
目的：观察转染白介素－１β－转换酶（ＩＣＥ）基因对血管平滑肌细胞的促凋亡作用。方法；通过阳离子脂质体介导的方法将重组质粒及报告质粒ＰＳＶ－β－ｇａｌ共转染体外培养的血管平滑肌细胞，运用生化染色、电镜观察、流式细胞仪及ＤＮＡ凝胶电泳等检测方法了解细胞转染后的凋亡发生情况。结果：ＩＣＥ转染阳性的细胞具有凋亡的典型形态学变化，且试验组的凋亡百分率较正常对照组及空白质粒对照组显著升高。结论：ＩＣＥ基因可诱导体外培养的平滑肌细胞发生凋亡。
Objective: To investigate the effects of apoptosis-induced gene-interlukin-1β-converting enzyme (ICE ) on cultured vascular smooth muscle cells (SMCs ). Methods: Using the cationic liposome mediation method, recombinant plasmid PCI-ICE was transfected into cultured vascular SMCs. Apoptosis was detected by biochemical staining, transmission electron microscopy, flow cytometry and gel electrophoretic analysis of DNA. Result:Gene transfected vascular SMCs displayed morphologic changes consistent with those of apoptosis. The incidence of apoptosis in gene transfected group was significantly higher than that of control group. Conclusion: ICE gene could effectively induce apoptosis in cultured vascular SMCs.
出处
《高血压杂志》
CSCD
2000年第1期64-67,共4页
Chinese Journal of Hypertension
关键词
白介素-1
β-转换酶
血管平滑肌细胞
凋亡
interlukin-1β-converting enzyme (ICE ), vascular smooth muscle cells, apoptosis
