摘要
探讨五福心脑清对实验性早期动脉粥样硬化大鼠组织形态学的影响。采用高脂乳剂制备实验性早期动脉粥样硬化大鼠模型,同时分别采用低、中、高不同剂量的五福心脑清(0.31、0.62、1.24 g/kg)以及非诺贝特(20 mg/kg)进行干预,实验10周后,检测实验动物血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋-胆固醇(LDL-C)、丙二醛(MDA)的含量、超氧化物歧化酶(SOD)活性,制备大鼠动脉组织病理切片及超薄切片在光镜及电镜下观察病理变化。结果发现五福心脑清不同剂量组给药10周后,与模型组相比,其血清的TC、TG、MDA等水平均有显著降低(P<0.01),HDL-C水平、SOD活性显著升高(P<0.05、0.01)。光镜观察及电镜表明,给予五福心脑清10周后大鼠早期动脉粥样硬化组织损伤明显减轻,五福心脑清中剂量组(0.62 g/kg)变化最为显著。表明五福心脑清对实验性早期动脉粥样硬化大鼠具有较好的预防和治疗作用,其机制可能是通过调节血脂、降低脂肪在动脉壁上的沉积以及清除氧自由基,抑制脂质过氧化产物的产生有关。
The objective of this study was to discuss the effects of WuFu Xinnaoqing （WFXNQ） on blood liqid, oxygen free radical and histomorphology in rat model with experimental early atherosclerosis. The rat model was induced by feed-ing lipid emulsion. The experimental rats were then garaged with low,medium and high doses of WFXNQ （0.31,0.62 and 1.24 g/kg） and fenofibrate （20 mg/kg） for 10 weeks followed by the detection of the total cholesterol （TC） ,tri-glycerides （TG）, high density lipoprotein cholesferol （HDL-C） ,low density lipoprotein cholestorol （LDL-C）, MDA, su-peroxide dismutase （SOD） activity in rats＇ serum of each groups. In addition,the tissue slices and super thin slices of rat carotid artery were prepared and observed under light microscopy and electron microscopy for pathological changes. The results showed that compared with model group, the serum TC, TG and MDA Levels of WFXNQ treatment groups were significantly reduced （ P 〈 0.01 ）. The HDL-C level, SOD activity of sample groups were significantly higher that these of the model group （P 〈0.05,0.01 ）. The light microscopic and electron microscopic observation results showed that the injury of rat tissues in WFXNQ treatment groups were significantly reduced compared with that of control group, in which the medium dosage of WFXNQ treatment group （0.62 g/kg） showed the most significant change. Based on the above results,it was concluded that WFXNQ had preventive and treatment effects on experimental early atherosclerosis by regulating blood fat, lowering fat deposition on artery walls, scavenging oxygen free radicals and inhibiting the genera-tion of lipid peroxidation products.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第4期504-508,538,共6页
Natural Product Research and Development
