摘要
目的：建立血清中氨茶碱定量分析的紫外分光光度法，探讨对氨茶碱中毒患者实际应用的价值，为临床氨茶碱中毒提供诊疗依据。方法取0．5 mL血清加0．2 mL的0．1 mol／L盐酸溶液，再加入三氯甲烷：异丙醇（95：5）的萃取液4．0 mL。充分震荡萃取，高速离心，取有机层于另一试管中加0．1 mol／L氢氧化钠溶液2．0 mL混匀反提，高速离心后取氢氧化钠层50μL紫外扫描。结果血清中氨茶碱最大吸收峰在（275±1 ）nm处，浓度在5．0～60μg／mL范围内呈线性，回归方程为Y＝0．0101X＋0．0065，相关系数（r）＝0．9998，回收率为85．5％～102．4％，相对标准差（RSD）为3．5％～4．8％。日内、日间RSD分别为3．08％～5．00％和3．16％～4．95％，最低检出浓度2．0μg／mL。结论该方法操作简便、分析快速、结果准确。为临床诊断氨茶碱中毒提供了一个简便准确的检测方法。
Objective To establish a quantitative analysis of serum theophylline UV spectrophotometry,aminophylline poisoning patients to explore the value of practical application,provide the basis for clinical diagnosis and treatment of theophylline poisoning. Methods 0.2 mL of 0.1 mol/L hydrochloric acid solution was put into 0.5 mL serum,then added 4.0 mL extract liquid. 50 μL of sodium hydroxide layer was detected by UV scanning after high-speed centrifugation. Results The results of serum theophylline absorption maxima was （275±1） nm,concentration of 5.0-60 μg/mL range linear regression equation was Y=0. 0101X＋0. 0065 （r= 0. 999 8）. The recovery rate was from 85. 5%to 102. 4% and the relative standard deviation （RSD） was from 3. 5% to 4.8%. The intra-day and inter-day RSD were 3.08%-5.00% and 3.16%-4.95%. The minimum detectable concentration was 2 μg/mL. Conclusion This method is simple,rapid,accurate results for detecting aminophylline poisoning.
出处
《国际检验医学杂志》
CAS
2014年第7期890-891,共2页
International Journal of Laboratory Medicine
关键词
血液
氨茶碱
中毒
紫外分光光度法
blood
aminophylline
poisoning
ultraviolet spectrophotometry
