摘要
目的:探讨天灸调节哮喘大鼠免疫失衡的机制。方法:将70只SD雄性大鼠分为正常组、安慰剂组、地塞米松组、大饼长程贴药组、大饼短程贴药组、小饼长程贴药组、小饼短程贴药组,每组各10只,除正常组外均采用卵白蛋白致敏和激发建立大鼠哮喘模型,对贴药组进行天灸治疗,其中大饼贴药组用1cm×1cm的大药饼贴药,小饼贴药组用0.5cm×0.5cm的小药饼贴药,每次按照相关穴位进行贴药,每次贴药5h,每天1次,共4次为一疗程。长疗程组共治疗3个疗程,短疗程组治疗1个疗程。地塞米松组予地塞米松进行腹腔注射,疗程与长程贴药组相同。实验结束后,观察哮喘大鼠外周血中嗜酸性粒细胞(EOS)变化;用酶联免疫吸附法(ELISA)检测大鼠肺组织中免疫球蛋白E(IgE)、细胞因子白介素4(IL-4)及干扰素γ(IFN-γ)的含量变化;用实时荧光定量(real-time Q-PCR)技术检测肺组织转录因子T-bet(T-box express in T cell)及GATA-3(GATA binding protein 3)蛋白的表达水平。结果:与正常组比较,安慰剂组全血EOS计数及血清IL-4、IgE含量均升高(均P<0.01),血清中IFN-γ含量明显降低(P<0.01));天灸贴药各组及地塞米松组可显著降低哮喘大鼠EOS、IgE水平及IL-4含量(均P<0.01),提高IFN-γ的含量(P<0.01)。与正常组比较,安慰剂组中T-bet mRNA表达显著降低(P<0.01);各治疗组能显著提高T-bet mRNA的表达,明显减少GATA-3mRNA的表达(P<0.01)。与短程贴药组比较,长程贴药组及地塞米松组中T-betmRNA的表达明显升高(P<0.01),GATA-3mRNA的表达显著降低(P<0.01);长程贴药组与地塞米松组比较无显著性差异(P>0.05),大饼贴药组与小饼贴药组比较亦无显著性差异(P>0.05)。结论:天灸能明显减轻哮喘大鼠的气道炎症,随时间的延长,疗效增加,具有明显的时效性,与药饼的大小无明显关系。
asthma rats. Objective To explore the mechanism of natural moxibustion on regulating immune imbalance in Methods Seventy SD male rats were divided into a normal group, a placebo group, a dexamethasone group, a big-cake for long-course moxibustion group, a big-cake for short-course moxibustion group, a small-cake for long-course moxibustion group and a small-cake for short-course moxibustion group, ten rats in each one. The rat model of asthma was established by egg albumen sensitization and stimulation in all the groups except the nor- mal group. The natural moxibustion was used in all moxibustion groups, in which big cake of 1cm×1cm size was used in the big-cake groups and small cake of 0.5 cm× 0.5 cm size was used in the small cake groups. According to relevant acupoints, the natural moxibustion was performed, 5 h per time, once a day. Four times of treatment was considered one course, and three courses were required in the long-course groups and one course was required in the short-course groups. Intraperitoneal injection of dexamethasone was applied in the dexamethasone group, which had the same course as long-course moxibustion group. After the treatment, changes of EOS in peripheral blood of asthma rats were observed; enzyme linked immunosorbent assay （ELISA） was applied to test the contents of IgE, IL-4 and IFN-γ in the lung tissue; real-time Q-PCR method was adopted to measure the expression level of transcription factor T-bet and GATA binding protein 3 （GATA-3） in the lung tissue. Results Compared with the normal group, the EOS in whole blood as well as IL-4 and IgE in plasma were all increased in the placebo group （all P〈 0.01）, IFN-γ in plasma was obviously decreased （P〈0.01）; while the levels of EOS, IgE and IL-4 were signifi- cantly reduced （all P〈0.01）, the content of IFN-γ was increased （P〈0.01） in all moxibustion groups and dexa- metnasone group. Compared with the normal group, the expression of T-bet mRNA in the placebo group was sig- nificantly reduced （P〈0.01）. Each treatment group could significantly increase the expression of T-bet mRNA and reduce that of GATA-3 mRNA （P〈0.01）. Compared with the short-course moxibustion groups, the expres- sion of T-bet mRNA was obviously increased in the long-course moxibustion group and dexamethasone group （both P〈0.01）, and that of GATA-3 mRNA was reduced （P〈0.01）. There was no significant difference be- tween long-course moxibustion group and dexamethasone group （P〉0. 05）, and also no significant difference could be seen between big-cake moxibustion group and small-cake moxibustion group （P〉0.05）. Conclusion The natural moxibustion could obviously reduce airway inflammation in asthma rats. With time passing, the efficacy is enhanced, indicating evident timeliness, which has no apparent relationship with the size of moxibustion cake.
出处
《中国针灸》
CAS
CSCD
北大核心
2014年第4期379-383,共5页
Chinese Acupuncture & Moxibustion
基金
广东省自然科学基金项目:S 201101000562
关键词
哮喘大鼠
天灸
细胞因子
转录因子
量效
时效
asthma rat
natural moxibustion
cytokines
transcription factors
dose effect
timeliness
