摘要
为解决现有的毛织物中甲醛检测方法耗时长、准确度不高的问题,建立了一种快速、灵敏地测定毛织物中甲醛含量的方法.使用0.1％的十二烷基磺酸钠溶液作为萃取溶剂,在30℃超声萃取15 min后,用乙腈-磷酸盐缓冲液（pH=2）配制的2,4-二硝基苯肼溶液作为衍生化试剂,超声辅助衍生化15 min,再用带有紫外检测器的高效液相色谱仪进行检测,流动相为V（乙睛）∶V（水）=60∶ 40,流速为1.0 mL/min,检测波长为350 nm.结果表明：甲醛加标回收率为81.36％ ～ 109.80％,相对标准偏差为2.20％ ～ 9.34％,定量限≤5.0 mg/kg.该方法具有前处理时间少,重复性好,结果准确等优点,为毛织物中甲醛的测定和相关质量安全评价提供了可靠手段.
A novel method was developed for rapid and sensitive analysis of formaldehyde in wollens samples to solve the problems of time-consuming and inaccuracy in the conventional test methods of formaldehyde in wollens samples. The wollens were extracted by 0. 1% sodium dodecyl sulfate solution under ultrasonic wave for 15 rain at 30 ℃ , then DNPH solution and prepared from acetonitrile-phosphate buffer （pH = 2） as the derivatization reagent, and the ultrasonic assisted derivatizations were processed for 15 min. The formaldehyde was determined by HPLC with ultraviolet detector with acetonitrilewater （60： 40, V/V） as mobile phase,at a flow rate of 1.0 mL/min and the wavelength of 350 nm. The result showed the recoveries were 81.36% - 109.80% , the relative standard deviations were 2.20% - 9.34% , and the limit of quantification was ≤5.0 mg/kg. This method had the advantages of less time for pretreatment, better reprodicibility and higher accuracy than conventional methods. It could be used to monitor the levels of formaldehyde in wollens samples for safety quality evaluation.
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2014年第4期66-70,共5页
Journal of Textile Research
基金
国家自然基金青年基金资助项目(31101702)
中央级公益性科研院所基本科研业务费专项资金项目(1610322014014)
关键词
甲醛
超声萃取
高效液相色谱
柱前衍生化
formaldehyde
ultrasonic extraction
high performance liquid chromatography
precolumn derivatization
