摘要
目的 :内皮细胞是一个非造血细胞 ,表达和 (或 )产生大多数已知的造血受体和细胞因子。这些因子和受体的生物学作用仍不十分清楚。本研究旨在探求内皮细胞产生的与造血有关的细胞因子GM -CSF及其生物学功能。同时对GM -CSF在造血调控中的作用及意义进行讨论。方法 :在建立人脐静脉内皮细胞体外培养模型的基础上 ,收集原代培养内皮细胞 1～ 9d培养上清液 ,离心 ,- 2 0℃保存备用。采用双抗夹心ELISA法对上清液中GM -CSF进行定性和定量测定。结果 :在未加任何刺激因素的条件下 ,从培养的人脐静脉内皮细胞培养上清液中可测及GM -CSF(35 0 794± 12 .10 95 pg/ml)。随着内皮细胞培养天数的增加 ,GM -CSF的释放量也随之增加。在第 6天时接近释放峰值GM -CSF(6 3 0 19pg/ml)。 结论 :体外培养的人脐静脉内皮细胞具有自发分泌GM -CSF的功能。GM -CSF能够促进粒系、红系和巨核系克隆形成。本研究为内皮细胞支持各系造血干、祖细胞的增殖和分化提供了理论依据。
Objective:Endothelial cells which are nonhemopoietic cells express and /or secrete most of the hematopoietic receptors and cytokines, whose biological role is still poorly understood. To search the biological action of GM-CSF, which was produced by endothelial cells on regulation of hematopoiesis.Methods:Human umbilical vein endothelial cells culture model was founded. The human endothelial culture supernatant(HECS) which was harvested from primary culture medium for 1～9 days and was centrifuged and stored at -20℃. GM-CSF in HECS was measured using sandwich enzyme-linked immunosorbent assay(ELISA) method. Results:GM-CSF was tested (GM-CSF:35.0794±12.1095?pg/ml) in HECS with no any stimulated factors on endothelial cells culture. The quantity of GM-CSF increases along with the cell culture time. At 6th day the quantity reaches its peak (63.019pg/ml). Conclusion:Human umbilical vein endothelial cells cultured in vitro can secrete GM-CSF spontaneously. GM-CSF can promote the clonic formation of granulocytic, erythrocytic, megakaryocytic and multipotential stem cells and progenitor cell.
出处
《内蒙古医学院学报》
2000年第4期220-222,共3页
Acta Academiae Medicinae Neimongol
