摘要
目的研究内皮抑素对体外培养的人脐静脉内皮细胞分泌IL-6和IL-8的抑制作用.方法将人脐静脉内皮细胞分为自然分泌组和实验组进行体外培养,收集第1-9天的培养上清液,采用双抗夹心ELISA法对上清液中IL-6和IL-8进行定量测定.结果在自然分泌组人脐静脉内皮细胞培养上清液中检测出IL-6和IL-8,证实了在自然状态下内皮细胞能产生一定量的IL-6和IL-8,且随着内皮细胞培养天数的增加,IL-6和IL-8的分泌量也随之增加,当细胞进入对数增长期后,IL-6和IL-8接近分泌峰值,分别为(29793.2±19.65)pg/ml和(6018.87±56.74)pg/ml.加入内皮抑素的实验组培养上清液中的IL-6和IL-8水平较自然分泌组差异显著(p<0.01).结论内皮抑素能够很好地抑制内皮细胞的生长和增殖并降低内皮细胞生物活性.
Objective To investigatetheinhibitoryeffectsexcercisedby endostatinon theproductionof interleukin-6(IL-6)andIL-8by humanumbilicalveinendothelialcells(HUVECs).Methods HUVECswereisolatedandculturedin vitro with endostatin(treatedgroup)or PBS(controlgroup),and thesupernatantwas harvestedfromtheprimaryculturemediumdaily for9consecutivedaysstartingfromthe firstday of culture,followedby centrifugation.IL-6and IL-8contentsin the supernatantweremeasuredusingsandwichenzyme-linkedimmunmosorbentassay(ELISA).Results IL-6andIL-8were detectedinthesupernatantof thecontrolcellculture,andtheiramountsincreasedas thecellculturewasprolonged,reaching thepeaklevelson day6(2979.32±19.65pg/mland6018.87±56.74pg/ml,respectively).In thetreatedgroup,however,the amountsof IL-6andIL-8weresignificantlylowerthanthecontrollevels(P<0.01).Conclusion Endostatincaninhibitthe growthandproliferationof endothelialcells,reducingtheirbiologicalactivities.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第1期54-56,共3页
Journal of First Military Medical University
