摘要
目的:探讨3种治法代表方剂对呼吸道合胞病毒(RSV)诱导引起肺炎的小鼠血清IL-6、IL-8及肺组织Toll样受体4(TLR4)、细胞核转录因子(NF-кB)的影响,揭示其治疗机制。方法:将Wister小鼠随机分为正常组、模型组、利巴韦林组、银翘散组、玉屏风散组、银屏散组。于感染前3 d,玉屏风散组、银屏散组连续给予玉屏风散(7.5 g·kg·d-1)3 d;除正常组以外,50μL 10 LD50病毒液滴鼻建立呼吸道合胞病毒感染的小鼠RSV肺炎模型。感染2 h后,银翘散组、银屏散组继续给予银翘散治疗(10 g·kg·d-1),连续给药3 d;采用ELISA法检测各组小鼠血清中IL-6、IL-8含量,免疫组化法检测分析肺组织TLR4、NF-кB P65蛋白的表达。结果:与正常组比较,RSV感染诱导小鼠肺组织中TLR4蛋白表达上调,增加NF-кB的活化(P<0.05),使血清中IL-6、IL-8水平升高(P<0.05)。银翘散组、玉屏风散组、双表法组测得IL-6、IL-8含量以及TLR4、NF-κB P65表达相较于模型组显著减弱(P<0.05)。与利巴韦林组比较,双表法组减弱尤为突出(P<0.05)。结论:3种治法对于治疗RSV肺炎均有显著疗效,尤其以双表法疗效更佳。其作用机制可能与抑制炎症因子IL-6、IL-8分泌及阻断TLR4/NF-κB信号通路有关。
Objective ： To investigate the influence of the representative prescriptions of three methods on expression of inflam- matory cytokines and toll - like receptor 4 （ TLR4 ） and nuclear factor KB （ NF -κB） in lung tissue of pneumonia mice models and to reveal the mechanism of the disease. Methods：48 Wister mice were randomly divided into 6 groups： normal group, model group, ribavirin group, Yinqiao Powder group, Yupingfeng group, Yinpin Powder group. Before 3 days of virus infection, Yupingfeng group and Yinpin Powder group were intragastrically given Yupingfeng at a dose of 7.5 g·kg- 1 . d - 1 by day 3. Except the normal group ,50 μL 10 LD50 RSV virus was intranasally inoculated to mice. 1 h after infection, mice in control and model groups were in- tragastrically given and mice in Yinqiao Powder and Yinping Powder group was intragastrically given Yinqiao Powder at a dose of 10 g·kg-1· d -1 by day 3. ELISA was used to the measure concentrations of IL -6 and IL - 8 in serum. Immunohistochemistry staining was used to detect the expressions of TLR4 and NF - κB P65 protein in lung tissue. Results ： Compared with the normal group, respiratory syncytial virus could up - regulate the expression of TLR4 protein and increase the activation of NF - κB P65 in lnng tissue（P 〈0. 05） ,and enhance the levels of IL-6 and IL-8 in mice serum. The levels of IL-2 and IL-4 were increased in Yinqiao Powder group, Yupingfeng group and Yinpin Powder group, compared with those of the model control group, there was a significant difference （P 〈 0. 05 ）. Compared with the ribavirin group, the expressions of those in Yinpin Powder group were weak- ened significantly. The expressions of those in Yinqiao Powder group were also lower than that of the ribavirin intragastric group（ P 〈 0. 05 ）. The decrease in Yupingfeng Powder group was not significant. Conclusion ： The three methods have a very significant effect on RSV pneumonia, especially the Shuangbiao method group（ P 〈 0. 05 ）. The mechanism may be associated with inhibition of production of inflammatory cytokine and the expression of TLR4/NF - κB signal transduction pathway.
出处
《辽宁中医杂志》
CAS
2014年第3期385-387,I0001,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81072975)
