摘要
以黄海黄杆菌 YS- 94 12 - 130突变株 SW1- 10 4为出发菌 ,在原生质体形成和再生的最佳条件下制备原生质体 ,对原生质体进行复合诱变 ,对大量再生突变株进行筛选和淡水驯化 ,最终获得了高产、稳定的碱性蛋白酶产生菌 SW2 - 10 4 ,在自来水培养基中能够大量产酶 ,产酶活力为 3910 U/ ml。从而解决了黄海黄杆菌 YS- 94 12 - 130低温碱性蛋白酶大规模工业化生产设备和产业化区域的局限性。
Taking the mutant F. yellowsea YS 9412 130 SW 1 104 as the initial strain, under the optimum conditions of the formation and regeneration,protoplasts of the strain are prepared, mutagenic treatments of the protoplasts are undergone. After selecting from a large amount of the regenerative mutants and culturing in fresh water, a stable mutant SW 2 104 producing high output alkaline protease is obtained. The strain can produce a large amount of enzyme in tap water medium, enzyme activity is 3 910U/ml. It is possible for low temperature alkaline protease from F. yellowsea YS 9412 130 to solve the problem of large scale production and to break the limitation of industrial region.
出处
《海洋水产研究》
CSCD
2000年第4期20-25,共6页
Marine Fisheries Research
基金
国家"海洋 86 3"项目 (86 3-819-0 6 -0 1)资助
关键词
黄海黄杆菌
原生质体
诱变
低温碱性蛋白酶
F. yelloweser Protoplasts Mutagenesis Low temperature alkaline protease
