摘要
通过2年大田试验，研究了化学杀雄剂GSC和SX1诱导白菜型冬油菜‘陇油6号’不育过程中花药呼吸脱氢酶活性、游离脯氨酸含量、蛋白质含量和保护性酶活性的变化。结果显示：（1）SX-1和GSC可以诱导白菜型冬油菜植株产生雄性不育，并以0．6mg／L左右GSC或9．0～10．0mg／L SX-1处理的不育株率最大，杀雄效果最好。（2）GSC和SX-1处理后，白菜型冬油菜花药呼吸脱氢酶活性显著降低；油菜叶片中游离脯氨酸含量增加，而花蕾中的游离脯氨酸含量降低；叶片中可溶性蛋白质含量无显著变化，花蕾中蛋白质含量降低；叶片中CAT和SOD活性增加，POD活性降低，而花蕾中CAT和SOD活性降低，POD活性增加。研究表明，化学杀雄剂处理后，白菜型冬油菜的花药呼吸脱氢酶活性、花蕾和叶片的游离脯氨酸、可溶性蛋白含量和保护酶活性发生了异常，并随着处理浓度的增加而加剧，导致体内活性氧清除代谢速度降低，细胞内膜脂过氧化作用增强，膜结构遭到破坏，花粉发育受阻，最终引起雄性不育。
Chemical hybridizing agents GSC and SX-1 ,were applied to study anthers breathing dehydrogen- ase activity, free proline content, protein content and protective enzyme activities of winter Brassica carnpestris ＇Longyou 6＇ for two years. The results showed that： （1）SX 1 and GSC can induce male sterility on B. campestris plants,and sprayed of GSC using 0.6 mg/L or SX-1 using 9.0～10.0 mg/L had the best inducing effect. （2）The anther breathing dehydrogenase activity was significantly decreased after GSC and SX-1 treatment;Free proline content in leaves increased,while decreased in buds;Protein content of leaves had no significant change, but protein content of buds decreased; CAT and SOD activities of leaves in- creased,POD activity decreased,while CAT and SOD activity of the flower buds decreased,POD activity increased. Research showed that anthers breathing dehydrogenase activity, free proline content, protein con- tent and protective enzyme activities of buds and leaves appeared exception after CHA treatment,and the a-nomaly became more significant with concentration increased,resulting in a lower metabolic rate of oxygen scavenging activity,the cell membrane lipid peroxidation enhanced and membrane structure destroyed,pol- len development blocked, eventually causing male sterility.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第3期516-522,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家现代农业产业技术体系(CARS-13)
国家行业科技项目(200903002-4)
国家863计划专项(2011AA10A104)
