摘要
采用聚丙烯酰胺凝胶电泳法，研究了具有甘蓝型油菜（ＢｒａｓｓｉｃａｎａｐｕｓＬ．）胞质、萝卜（Ｒａｐｈａｎｕｓｓａｔｉｖｕｓ）胞质的结球白菜同核异质雄性不育系─Ｐｏｌｃｍｓ３４１１－７、Ｏｇｕｃｍｓ３４１１－７及其保持系—３４１１—７不同发育时期、不同器官过氧化物酶同工酶谱的差异。结果表明，不育系与保持系过氧化物酶同工酶谱的差异主要在花蕾中，表现为不育系酶带多而且活性强，其它器官差异较小；Ｐｏｌｃｍｓ３４１１－７与Ｏｇｕｃｍｓ３４１１－７的区别在苗期、蕾期、花期及叶片、根和花蕾中都有表现；讨论了过氧化物酶同工酶的表达与雄性不育性及细胞质的关系。
The differences of peroxidase isoenzyme among the two allocytoplasm male sterile lines with the same nucleus (AA, Zn=20) (Polcms3411-7 and Ogucms3411-7) and separately by transferred ms of Brassica napus (Polima, Shiga, 1980) and ms of Raphanus sativus (Ogura, 1968) to Chinese cabbage (B. campestris ssp. pekinensis) and their common maintainer (3411-7, AA, 2n=20) at different developmental stages and organs were studied by using polyacrylmide gel electrophoresis, the main results obtained are as follows:The differences between the two CMS lines and their maintainer existed in the flower buds, especially in the bigger buds; there were more band numbers in the CMS lines than the maintainer and these bands were more active.The differences between Polcms3411-7 and Ogucms3411-7 existed in all the developmental stages and organs.
出处
《园艺学报》
CAS
CSCD
北大核心
1994年第3期264-268,共5页
Acta Horticulturae Sinica
基金
国家自然科学基金
关键词
结球白菜
白菜
细胞质
雄性不育系
Chinese cabbage
Cytoplasm male sterile line
Peroxidase isoenzyme
