摘要
目的探讨不同负荷游泳运动对大鼠学习记忆的认知能力及海马神经型一氧化氮合酶(n-NOS)表达与疲劳机制。方法将40只体重为350-400g的Wistar雄性大鼠分为对照组和每天进行15mins、30mins、60mins的小中大负荷游泳运动的实验组;实验持续8周后采用"Y迷宫"进行学习记忆能力测试;利用S-P免疫组化染色法检测海马神经n-NOS的表达水平。结果学习记忆的达标次数和错误次数,中等负荷组显著低于对照组和大负荷组(P<0.05和P<0.01);与对照组相比中、大负荷组海马神经n-NOS阳性表达均显著提高(P<0.01);与小负荷组比较,中负荷组和大负荷组均有显著性差异(P<0.05和P<0.01);中、大负荷组间也具有非常显著性差异(P<0.01)。结论中等游泳运动负荷能够有效的提高大鼠学习记忆能力,海马神经n-NOS表达水平随着运动负荷的增加而显著提高;大运动负荷组学习记忆能力下降而n-NOS表达水平提高,其相互作用机制有待于深入研究。
Objective The purpose is to explore the fatigue mechanism of rats＇ cognitive ability to learn and memorize and the expression levels of hippocampal neuron n-NOS under various swimming loads.Methods 40 Wistar male rats weighing 350-400g are divided into a control group and 3 experimental groups that swim under various loads everyday,for 15min,30min,and 60min respectively.After 8 weeks,the rats receive ability tests of learning and memorizing though a Y-maze and the levels of hippocampal neuron n-NOS expression are measured,by means of ummunity group dyeing.Results in the times of pass and failure,the group under moderate load is significantly lower than the control group and the group under large load （P＜ 0.05,P＜ 0.01）;compared with the control group,positive expression of hippocampal neuron n-NOS levels of the groups under moderate and large loads increase significantly（P＜0.01）;there is a significant difference between the group under small load and groups under moderate and large loads（P＜0.05,P＜0.01） ;there is also a significant difference between the group under moderate load and the one under large load（P＜0.01）.Conclusion swimming of moderate load can effectively improve rats＇ ability to learn and memorize; the levels of hippocampal neuron n-NOS expression increase significantly along with the increase of the loads; the ability to learn and memorize of the group under large load decreases while its levels of hippocampal neuron n-NOS expression increase,and mechanism of the interaction is to be further explored.
出处
《中国实验诊断学》
2014年第3期377-379,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅自然科学基金面上项目(201215037)
