摘要
目的探讨神经生长因子(NGF)对脑出血血肿灶周神经元和神经胶质细胞的影响.方法健康家犬28只,随机分为两组:NGF组(n=20):注血后0.5 h,将NGF 2000 AU立体定向导入血肿灶周区;脑出血(ICH)组(n=8):只注血,不注药.在ICH后1、3、10、28 d四个时间点进行以下检测:①采用Purdy评分观察临床神经功能恢复情况.②采用免疫组化SP法,检测外源性NGF在血肿灶周脑组织中的有效表达.③激光共聚焦显微镜检测血肿灶周神经元烯醇化酶(NSE)、神经纤维酸性蛋白(GFAP)荧光单标阳性细胞的数目.结果①1～3 d Purdy评分两组比较无显著性差异(P＞0.05),但10～28 d评分NGF组显著好于ICH组(P＜0.05).②NGF组血肿灶周免疫阳性细胞3 d时大量出现,染色较深.持续10 d,而ICH组表达量少且持续时间短(P＜0.05).③NGF组激光共聚焦显微镜下NSE、GFAP单标阳性细胞数均明显多于ICH组(P＜0.05).结论外源性NGF立体定向导入血肿灶周区,早期能够通过保护神经元提高存活率;晚期能够通过促进胶质细胞适度增生、神经纤维生长来促进血肿灶周神经功能的修复.
Objective To study the effect of NGF on neuron and gliacyte in the region of perihematoma after experimental intracerebral hemorrhage. Methods 28 healthy dogs were divided into two groups at random. NGF group（ n = 20）： NGF （2000AU） were given in the region of perihematoma at 0.5 h after ICH; ICH group（ n = 8） ：only blood was injected. The following examinations were carried out at 1, 3, 10, 28 d after ICH：①The lesioned degree of neurological function was assessed by Purdy score. ②Immunohistochemistry SP method was adopted to detect whether exogenous NGF could be effectively expressed in the region of perihematoma or not. NGF immune positive cells were stained brown - yellow colour in the cytoplasm. ③ LSCM detected the number of NSE and GFAP immunolluorescence positive cells. Results ①There were no significant differences in the Purdy score between the two groups 1 - 3 d （ P 〈 0.05）, but there were significant differences from 10 d to 28 d （ P 〈0.05）. ②In NGF group, the positive cells in perihematoma were scattered densely and stained thickly, which kept for 10 days. In ICH group, the positive cells in perihematoma were scattered thinly and stained lightly （ P 〈 0.05）. ③The NSE and GFAP in NGF group were higher than those in ICH group, which had significant difference （ P 〈 0.05）. Conclusions The exogenous NGF can accelerate the neurological functional recovery after ICH by protecting neuron and increasing survival rate at the early stage, and stimulating gliacyte proliferation, nerve fiber growth at the late stage.
出处
《中国急救医学》
CAS
CSCD
北大核心
2005年第9期651-653,共3页
Chinese Journal of Critical Care Medicine
基金
河北省自然基金资助项目(No.C2004000590)
