摘要
用棉花胚珠切块诱导愈伤组织 ,经悬浮振荡培养、漂浮培养、滤纸桥法等方法诱导成纤维细胞。发现漂浮培养和改进的滤纸桥法对纤维的诱导效果比悬浮振荡培养的效果好。微管解聚剂 APM和核酸抑制剂抑制纤维的生长 ,纤维二糖有一定的促进作用。
Cotton fibers were induced from cotton ovule callus cells by suspension culture, float culture or filter paper bridge culture. It was found that float culture or filter paper bridge culture was better than suspension culture. APM and actinomycin D inhibit the fiber elongation. Cellobiose promotes its development. Polarity affects its culture.
出处
《作物学报》
CAS
CSCD
北大核心
2000年第4期496-500,共5页
Acta Agronomica Sinica
基金
国家自然科学基金资助项目(编号39670047).
关键词
棉胚珠
愈伤组织
纤维诱导
极性
Cotton ovule
Callus
Cotton fiber induction
Polarity
