摘要
用酶联免疫法分别测定了开花前３ｄ、１ｄ，开花当天，开花后１、３、５、７、８、１２ｄ的５个棉纤维突变体胚珠中ＧＡ１＋３、ＩＡＡ、ＡＢＡ及ｉＰＡｓ的含量。结果表明，５个纤维突变体内源ＩＡＡ差异主要发生在开花受精前；内源ＧＡ１＋３差异主要表现在ＧＡ１＋３增加速率最大的时期，对照ＧＡ１＋３增加速率最大的时期是在受精前，而５个突变则在受精后；内源ｉＰＡｓ及ＡＢＡ的差异主要表现在峰值出现时期上，有极短纤维和正常短绒的突变体Ｌｉ与对照品种一样ｉＰＡｓ峰值出现在受精前，ＡＢＡ峰值则在受精后，而无短绒有纤维和无短绒无纤维的突变体则相反。发现了突变体ＬｉｇｏｎＬｉｎｔｌｅｓｓ纤维极端缩短的原因主要是受精后出现了极高含量的ＡＢＡ。指出了内源ＧＡ１＋３和ＡＢＡ则分别在受精后促进和抑制胚珠增重和纤维伸长。
The contents of IAA、GA1+3、ABA and iPAs of cotton ovules colleced on -3,-1,0,+1,+3,+5,+8,+12 days after anthesis in 5 types of Lint and fuzz mutants were examined by using Enzyme Linked Immuno Sorbent Assay(ELISA). The results indicated that the difference of endogenous IAA among 5 fiber mutants occurred before anthesis. The difference of endogenous GA1+3 could be seen when GA1+3 increasing rates(GIR)were dramatically high. The period of highest GIR of check variety were before fertilization while that of 5 mutants were after fertilization. The difference of endogenous ABA and iPAs mainly occured during the peoriod of peak value of these two hormones. Like that of the check variety with normal fuzz and lint, the peak value of iPAs of Ligonlintless, with normal fuzz and very short lint, occured before fertilization, and its ABA peak value was shown after fertilization. However, the peak value of iPAs and ABA of the mutants of fuzzless with normal lint or lintless were reversed. It was discussed that endogenous GA1+3 and ABA, respectively advance and inhibit the increasing of ovule weight and fiber elongation after fertilization. It was found that the dramatically high content of ABA after fertilization led to extremely short lint of Ligonlintless.
出处
《棉花学报》
CSCD
北大核心
1998年第1期43-51,共9页
Cotton Science
关键词
棉花
突变体
纤维
生长素
赤霉素
脱落酸
生长
cotton mutant fiber indoleacetic acid gibberellic acid abscisic acid kinetin
