摘要
在 4种不同的培养程序中应用了几种处理方法 ,并对其在诱导水稻原生质体起源的愈伤组织再生植株中的效果进行了比较。直接将愈伤组织从含有 2 ,4 - D的增殖培养基转移到含有 BA和 NAA的植株再生培养基上培养 ,只能得到少量的弱苗 (第 1种程序 )。在增殖培养基中添加 ABA诱导了结节状的愈伤组织形成 ,使愈伤组织的植株再生能力明显加强 (第 2种程序 ) ;而在植株再生培养基中添加ABA则使愈伤组织变得紧结并形成生长受抑制的不定芽 ,当这些愈伤组织被转移到不含 ABA的生长培养基后 ,不定芽开始快速生长 (第 3种程序 )。先将愈伤组织培养在含有 ABA的增殖培养基上 ,然后相继转移到含有 ABA的植株再生培养基和生长培养基上 ,可以取得大量健壮的再生苗 (第 4种程序 )。统计结果显示 ,第 2和第 3种程序的培养效果比第 1种程序要好 ,而第 4种程序的培养效果则比其他程序好得多。
Four different culture procedures combined with several treating methods were compared for their effects on regeneration of plants from protoplast derived calluses of rice. Only a small number of weak plants could be regenerated when the calluses were transferred and cultured directly from a proliferation medium containing 2, 4 D to a plant regeneration medium containing BA and NAA (procedure 1). Addition of ABA to the proliferation medium induced the formation of nodular calluses with enhanced regeneration potential (procedure 2), while addition of ABA to the regeneration medium resulted in the formation of compact calluses with suppressed adventitious buds, which would grow fast upon transfer to a growth medium free of ABA (procedure 3). By Culturing of the calluses consequently on ABA supplemented proliferation medium, ABA supplemented regeneration medium and the growth medium (procedure 4), large number of more healthy plants was obtained. Statistical test indicates that procedure 2 and 3 were much more efficient than procedure 1, while procedure 4 was the most efficient for plant regeneration.
出处
《作物学报》
CAS
CSCD
北大核心
2000年第4期490-495,共6页
Acta Agronomica Sinica
基金
This research was supported by The Chinese Foundadon for Agricultural Science and Education.
