摘要
目的探讨1L-27对支气管哮喘（简称哮喘）患者干扰素y（lFN-γ）及1L-33的调节作用。方法选择56例非细菌性感染所致中-重度哮喘患者为试验病例组，整群匹配方法选择30名正常者为对照组，酶联免疫吸附试验法检测1L-27、干扰素y（IFN-y）、1L-33水平，全血细胞分析仪检测白细胞数、中性校细胞绝对值、嗜酸粒细胞绝对值;同时收集哮喘组患者外周血单个核细胞（PBMC）.加入不同浓度的（5μgiL或0.5μgiL）重组人白介素27（rh1L-27）进行体外培养12 h.酶联免疫吸附试验法检测其培养 上清1FN-y及1L-33浓度;探讨1L-27对1FN-γ、1L-33的影响及各指标间的相关性。结果哮喘组患者血清1L-33较对照组明显升高（t=34. 53. P 〈0. 05）.哮喘组1FN-γ、1L-27水平较对照组明显降低 （ t IFN-γ=27.52. P IFNγ〈0.01; t IL-27 = 32.77 ？ P IL-27 〈0.01） ;不同浓度的rh1L-27刺激PBMC后进行体 外培养后.IFN-y表达分别为[（85.9±12. 1）μgiL.（19.6± 3.2）μgl日，较细胞剌激素组（11. 3±2. 7）μgiL 均有显著升高（t = 34.57. P 〈0. 01; t = 11. 39. P 〈0. OIL 5μgiL rh 1L-27组1L-33表达较对照组有 明显降低[（33.2士11.8）μgiL vs 016.9土17.的μgiL. t =22.69. P 〈0. 01];哮喘患者1FNγ水平变 化与rh1L-27应用剂量呈正相关性（ r = 0. 949. P 〈 0. 0日，而rh 1L-27与1L-33呈负相关性（r = 0.89. P 〈0. 05）。结论 1L-27、1FN-y、1L-33表达水平间存在一定关联强度。
Objective To study the effect of interleukin （IL）-27 on INF-y and IL-33 activities in patients with bronchial asthma （asthma）. Methods Two groups of experiments, the serum levels of IFN-y and IL-27 and IL-33 in asthmatic patients or control group by ELISA. The levels of WBC and neutrophils and EOS by hematology analyzer. PBMCs from 33 asthmatic patients were cultured with recombinant human IL-27 （5 μg/L or 0. 5 μg/L） for 12 hours in vitro, and then the levels of IFN-y and IL-33 in their supernatant were measured by ELISA. The explore of correlation between IL-27 activating factor and IL-33 , IFN-y in the asthmatic patients. Results The levels of IL-33 in asthmatic patients were significantly increased and IFN-y, IL-27 had a significant decrease compared with that in healthy control group （t IFN-y=27. 52, P IFN-y〈0. 01; t IL-27 =32.77, P IL-27 〈0. 01）. The values of IFN-y in supernatant were （85.9 ± 12.1） flg/L, （19. 6 ± 3.2） μg/L compared with that in cytokine stimulated control group （11. 3±2. 7） μg/L had increased significantly （ t = 34.57, P 〈0. 01; t = 11. 39, P 〈0. 01）. The group treated with 5 μg/L IL-27 had decreased significantly IL-33 levels compared with the cytokine stimulated control group [（33. 2± 11. 8） μg/L vs （116. 9± 17.6） μg/L, t = 22. 69, P 〈0.01]. There was a positive correlation between IFN-y and IL-27 in asthmatic patients. Conclusions The levels of IL-27 ,IFN-y, IL-33 were interelated.
出处
《国际呼吸杂志》
2014年第5期321-324,共4页
International Journal of Respiration
基金
2013年湖北省自然科学基金指导性项目（2013CFC057）
黄石市医药卫生科研项目（2013-34）
