摘要
目的：建立HPLC法同时测定并比较南方红豆杉与德国引种曼地亚红豆杉茎、叶中紫杉醇和10-脱乙酰巴卡亭Ⅲ含量。方法采用CosmasilTMC18色谱柱（250mm×4．6mm，5μm）；以乙腈-水（50∶50）为流动相；流速为1．0mL·min-1；检测波长为227nm；柱温：30℃。结果紫杉醇、10-脱乙酰巴卡亭Ⅲ的线性范围分别为2．675～42．8μg·mL-1（r＝0．9993）和3．875～62μg·mL-1（r＝0．9996），平均加样回收率（n＝9）分别为98．1％和101．6％，RSD分别为2．7％和2．3％。南方红豆杉与德国引种曼地亚红豆杉的茎、叶中紫杉醇含量分别为0．12和0．10mg·g^-1，10-脱乙酰巴卡亭Ⅲ含量分别为0．12和0．14mg·g^-1。结论引种红豆杉与南方红豆杉在同一地区的生态环境下生长，茎、叶中所含紫杉醇和10-脱乙酰巴卡亭Ⅲ含量有显著性差异（P＜0．05）。所建立方法准确，简便，适用于红豆杉的质量评价，为不同栽培品种的选择提供参考。
Objective To determine and compare the contents of taxol and 10-deacetyl bacatin Ⅲ in stems and leaves between Taxus chinensis mairei and Taxus x media by HPLC .Methods The isolation was performed on a CosmasilTM C18 column（250 mm ＆#215; 4 .6 mm ,5 μm） with the mobile phase consisting of acetonitrile-water（50∶50） .The flow rate was 1 .0 mL ＆#183; min-1 and the column temperature was set at 30 ℃ .Results The standard curves were linear within the ranges of 2 .675-42 .8 μg ·mL -1 for taxol（r=0 .999 3） ,and 3 .875-62 μg · mL -1 for 10-deacetyl bacatin Ⅲ （r=0 .999 6）;The average recoveries were 98 .1% and 101 .6% , RSD were 2 .7% and 2 .3% .The contents of taxol in Taxus chinensis mairei and Taxus x media were 0 .12 and 0 .10 mg ＆#183; g -1 , respectively ,while 10-deacetyl bacatin were 0 .12 and 0 .14 mg ＆#183; g -1 ,respectively .Conclusion Although growing in the same eco-logical environment ,significant differences（P〈0 .05） of the contents of taxol and 10-deacetyl bacatin Ⅲ in stems and leaves were observed between Taxus chinensis mairei and Taxus x media .The method is simple and could be used as a quality control method for Taxus plants .
出处
《西北药学杂志》
CAS
2014年第2期129-132,共4页
Northwest Pharmaceutical Journal
