摘要
目地:观察视黄酸(retinociacdi,RA)对龟甲提取物(Extracts from Testudinis Carapacis et Plastri,PTE)促骨髓间充质干细胞(mesenchymal stem cells,MSCs)增殖的影响及其作用机制。方法:将PGL3-ID1用磷酸钙共沉淀法转染大鼠MSCs。PTE联合视黄酸(RA)、视黄酸受体阻断剂Ro41分别以10-6、10-7、10-8mol/L的浓度作用于转染后MSCs 36 h,萤光素酶报告基因检测ID1的表达。1、3、30、100μg/mL PTE分别作用于MSCs 36 h、3 d、7 d,RTPCR检测RAR的表达。PTE分别联合10-6mol/L RA、10-7mol/L Ro41作用于MSCs 36 h,RT-PCR检测RAR、ID1的表达。结果:PTE促进了MSCs中ID1的表达,联合应用RA,其促进作用增强,同时促进了RAR的表达;应用Ro41阻断RAR,PTE对ID1的促进作用减弱。结论:RA促进了MSCs中ID1的表达,PTE通过调控核受体RAR的表达水平来调控MSCs的增殖和分化。
Objective：To explore the effect of retinociacdi（ RA） combined extracts from Testudinis Carapacis et Plastri（ PTE） on proliferating in MSCs and its mechanism.Methods：Transfected PGL3-ID1 using the calcium phosphate co-precipitation method in rat MSCs.PTE combined with RA and retinociacdi receptor inhibitor（ Ro41） acted on transfected MSCs with respective concentrations of 10- 6,10- 7and 10- 8mol/L.Luciferase activity measurement was used to detect the activity of RAR and ID1 36 h later.PTE acted on MSCs 36 h,3 d and 7 d for respective concentrations of 1,3,30 and 100 μg/mL,then collected cells to detect RAR with RT-PCR.PTE combined with RA for 10- 7mol/L and Ro41 for 10- 6mol/L respectively on MSCs for 36 h,and then collected cells to detect RAR and ID1 with RT-PCR.Results：PTE promoted expression of ID1 on MSCs.When combined with RA,the promotion effect became greater and it promoted expression of RAR at the same time;When inhibited RA using Ro41,the promotion of ID1 was weaken by PTE.Conclusion：RA promotes expression of ID1 on MSCs,PTE regulates proliferation and differentiation of MSCs by expression of nuclear receptor RAR.
出处
《中药材》
CAS
CSCD
北大核心
2014年第1期87-90,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金资助项目(30772861)
肇庆市科技创新计划项目(2013E181)
关键词
视黄酸
龟甲提取物
骨髓间充质干细胞
核受体
Retinociacdi
Extracts from Testudinis Carapacis et Plastri
Mesenchymal stem cells
Nuclear receptor
