摘要
目的:观察龟板组份及配伍对体外培养骨髓间充质干细胞增殖的影响,筛选龟板组份中进MSC增殖的药效成分方法:从龟板提取物分离出10个组份,以不同浓度(2μl,5μl,10μl,20μl)组份及配伍加入体外培养的MSC中,培养3 d后,MTT法测定细胞活性。结果:与对照组比较,A、B、H、I 20μl浓度组和C、F(10μl～20μl)浓度组和J(5μl～0μl)浓度组细胞活性显著提高,与J组份配伍细胞活性提高(P<0．05);E(2μl-20μl)浓度组细胞活性显著降低,与E组份配伍组细胞活性显著降低;D和G组细胞活性没有影响。结论:7个组分均促进MSC增殖,以J组份最强,与J组份配伍作用提高;E组份抑制MSC增殖,与E组份配伍作用降低;D和G组份无效。
Objective ： To observe the effect of extract components from Plastrum Testudinis and their combination component on the proliferaiton of mesenehymal stem cell（MSC） to screen out the effective components. Methods： Mesenchymal stem cells were dissociated from bone marrow of rat and were marked by Brdu, and the expression of CD44, CD54 and double label of Brdu and CD44 extract components（A -J） with different concentration （2μl, 5μl, 10μl,20μl）added to cultured MSC. The cell viability was tested by MTT. Results： Cell viability in component A, B, H, I with 20 μl, C, F with 10μl and J with 5μl concentration group increased compared with that in control group（ P 〈 0. 05 ）and cell viability in compponent E with 2μl～20μl concentration groups decreased compared with that in control group（ P 〈 0.05）. Cell viability in component D and G with 2μl～20μl concentration groups was similar to that in control group. Cell viability in combination component with J component increased, but cell viability in combination component with E component decrased. Conclusion ： All compoents A, B,C, F, H,I and J can improve proliferation of MSC. Anong them, component J and its combination are the best. Component E and its combination can inhibit proliferation. But component D and G have no effect on proliferation of MSC.
出处
《中药材》
CAS
CSCD
北大核心
2006年第5期452-455,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(批准号30271677
30371837
30472272)
广东省自然科学基金项目(批准号31483)
广东省中医药管理局项目(批准号2050025)
关键词
龟板组份
骨髓间充质干细胞
细胞增殖
Extract components from Plastrum Testudinis
Combination component
Mesenchymal stem cell
Cell proliferation
