摘要
同源异型盒基因在决定植物的形态建成中发挥着重要作用。采用RT-PCR和RACE技术,从麻竹中分离到1个同源异型盒基因,其cDNA全长1 452 bp,编码1个330个氨基酸的蛋白,包含1个KNOX1区、1个KNOX2区、1个ELK区和1个Homeobox区,属于KNOX蛋白,该基因命名为DlKNOX1(GenBank No.KF709955)。组织表达特异性分析表明,DlKNOX1在节中的表达量最高,茎和鞘中次之,而在叶片中最低。将DlKNOX1置于35S启动子控制下,构建到载体pPZP的多克隆位点并转化拟南芥。RT-PCR分析表明,DlKNOX1已在转基因植株中表达。转基因植株表型变化明显,花期比野生型延迟,果荚着生部位明显发生变化,这意味着DlKNOX1基因可能参与麻竹形态建成的调控。
Homeobox genes play important roles in determining plant morphogencsis. A homeobox gene was isolated from Dendrocalamus latiflorus with RT-PCR and RACE methods. The full length of the cDNA was 1452 bp which would encode a 330-aa protein containing one KNOX1 domain, one KNOX2 domain, one ELK domain and one Homeobox domain, indicating that it belongs to KNOX protein, and the gene was des!gned as DIKNOXI. Tissue specific expression showed that D1KNOX1 expressed in knob with the highest level, followed by in stem and sheath, and the lowest in leaf. DIKNOX1 gene was constructed into the multiple cloning sites of pPZP vector driven by a 35S promoter, and transferred into Arabidopsis thaliana. RT-PCR analysis showed that DIKNOX1 was expressed in the transgenic plants. The phenotype of transgenics showed that flowering time was later than that of wild type, and the pods position chansed obviously, which suggested that DIKNOX1 might participate in the regulation of D. latiflorus morphogenesis.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2014年第2期56-62,共7页
Scientia Silvae Sinicae
基金
“十二五”国家科技支撑计划项目“竹子优良种质选育技术研究与示范”专题“分子辅助育种技术集成与示范”(2012BAD23B0504)
关键词
麻竹
同源异型盒基因
组织特异性表达
异位表达
形态建成
Dendrocalamus latiflorus
homeobox gene
tissue specific expression
ectopic expression
morphogenesis
