摘要
采用RT-PCR和RACE技术,从毛竹中分离到1个含完整的编码区和5′端非翻译区的cDNA,长776bp,编码240个氨基酸,命名为PeMADS1(GenBank登记号:EU327784)。序列分析表明,该基因具有典型的植物MADS-box基因结构,与拟南芥的E类功能基因AGL6编码蛋白的一致性为57.2%。将PeMADS1基因置于CaMV35S启动子控制下,构建PeMADS1基因植物表达载体。应用农杆菌介导将目的基因转入拟南芥,RT-PCR证实PeMADS1基因得到异位表达。转基因拟南芥呈现开花提早、莲座叶卷曲、发育迟缓等表型,表明PeMADS1可能参与毛竹由营养生长转向生殖生长的发育调控。
A cDNA containing an open reading frame and 5′ untranslated regions was isolated from Phyllostachys edulis by reverse transcription polymerase chain reaction（RT-PCR） using degenerate primers and by 5′ rapid amplification of cDNA ends（RACE） ,and named as PeMADS1（GenBank accession No. EU327784） . PeMADS1 was 776 bp and encoded a protein of 240 aa. The gene had a typical MADS-box gene structure. Homology analysis showed that PeMADS1 shared 57. 2% similarity with AGL6,indicating that it belongs to E function genes. The plant expression vector of PeMADS1 gene driven by CaMV 35S promoter was constructed and transferred into Arabidopsis thaliana. Ectopic expression of PeMADS1 was confirmed by RT-PCR. The transgenic plant showed different phenotype such as early flowering,curled rosette or stunt,which indicated that PeMADS1 might participate in regulating the flower development of P. edulis.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2010年第10期37-41,共5页
Scientia Silvae Sinicae
基金
"十一五"林业科技支撑计划专题(2006BAD19B0202)
林业公益性行业科研专项(200704001)
国家林业局重点林业科学技术研究项目(2007-01)
