摘要
目的:探讨实验性变应性鼻炎(llergic rhinitis,AR)发病机制中白介素(IL)-6、IL-17和免疫球蛋白E(IgE)水平的变化及其意义,分析消风宣窍汤对这3种细胞因子的调节作用。方法:用卵蛋白(OVA)通过腹腔注射结合滴鼻的方式建立AR豚鼠模型,造模周期共21 d,将60只Hartley豚鼠随机分为正常组、模型组、消风宣窍汤高、中、低剂量组(42,21,10.5 g·kg-1)、氯雷他定组(1.34 mg·kg-1)。用被动皮肤过敏试验(PCA)检验豚鼠模型制备情况,ig治疗1周,ELISA法检测各组血清中免疫球蛋白(IgE)和IL-6,IL-17的水平。结果:与正常组比较,模型组IgE和IL-6,IL-17水平明显升高(P<0.01);与模型组比较,消风宣窍汤高、中剂量组IgE水平显著降低(P<0.01),IL-6,IL-17水平降低(P<0.05或P<0.01);低剂量组IgE水平降低(P<0.05);氯雷他定组IgE水平降低(P<0.05),IL-6,IL-17水平显著降低(P<0.01)。结论:消风宣窍汤降低AR的IgE水平的机制可能是通过降低IgE和IL-6,IL-17水平实现的,并且两者呈正相关关系,提示IL-6和IL-17在AR发病机制中有重要作用。
Objective：To investigate the role of immunoglobin E （IgE） and interleukin-6 （IL-6）,interleukin-17 （IL-17） in the pathogenesis of allergic rhinitis （AR）,and the regulative effect of Xiaofeng Xuanqiao decoction on the above 3 cytokines.Method：AR guinea pig model was established by intraperitoneal injection and intranasal way with ovalbumin （OVA）,and modeling cycle is 21 days.Sixty guinea pigs were randomized into 6 groups：normal control group,AR group,high-dosage,middle-dosage,low-dosage Xiaofeng Xuanqiao decoction group （42,21,10.5 g ·kg-1）,and loratadine group （1.34 mg ·kg-1）.Passive cutaneous anaphylaxis （PCA） test was performed in all guinea pigs.Xiaofeng Xuanqiao decoction and loratadine were applied to intervene AR guinea pig.The serum levels of IgE and IL-6,IL-17 were determined by enzyme-linked immunosorbant assay （ELISA） method.Result：The expression levels of IgE and IL-6,IL-17 significantly increased compared with the normal group （P ＜0.01）.Compared with that in AR model group,the serum level of IgE in high-dosage Xiaofeng Xuanqiao decoction group significantly was decreased （P ＜ 0.01）,the levels of IL-6,IL-17 were decreased （P ＜ 0.05）; the levels of IgE and IL-6,IL-17 in equivalent-dosage Xiaofeng Xuanqiao decoction group significantly were decreased （P ＜ 0.01） ; the level of IgE in low-dosage Xiaofeng Xuanqiao decoction group decreased （P ＜ 0.05）,the levels of IL-6,IL-17 have no obvious changes ; the level of IgE in loratadine group decreased （P ＜ 0.05）,and the levels of IL-6,IL-17 significantly decreased （P ＜ 0.01）.Conclusion：Xiaofeng Xuanqiao decoction down-regulate serum level of IgE through decreasing the levels of IL-6 and IL-17,and there is a positive correlation between them,suggesting that IL-6 and IL-17 are important factors in the mechanism of AR.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第6期167-170,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏高校优势学科建设工程项目(苏政办发〔2011〕6号)
