摘要
目的了解长期留置管细菌分布及生物膜形成能力。方法 10%血清肉汤振荡培养留置管中细菌,常规生化和分子生物学方法鉴定分离菌,结晶紫半定量法检测分离菌生物膜形成能力。结果留置管细菌检出率为49.1%(53/108);共分离60株菌,以葡萄球菌分离率最高(70.0%),其次为肠球菌(15.0%)和大肠埃希菌(6.7%);所有分离菌均具有生物膜形成能力,其中38株(63.3%)具有较强的生物膜形成能力。结论长期留置管细菌检出率较高,大部分分离菌具有较强的生物膜形成能力。
Objective To reveal the distribution and biofilm forming ability of bacteria isolated from longtime in- dwelling tubes. Methods Bacteria from longtime indwelling tubes were enriched and shaking cultured in broth containing 10% calf serum, then isolated and identified by routine method and PCR. The biofilm formation capa- bility of each isolated bacterium was detected by crystal violet semi-quantitative method. Results The bacterial detection rate of the longtime indwelling tubes was 49.1% （53/108）. Among the 60 strains of bacteria isolated, the most frequently occurred bacteria was Staphylococcus （70%） , followed by Enterococcus （15.0%） and Esche- richia coli （6.7%）. All the strains isolated in this study had biofilm forming ability, 38 （63.3%） of which had strong capabilities of biofilm formation. Conclusion The bacterial detection rate of longtime indwelling tubes is remarkably high and most isolates have strong biofilm forming abilities.
出处
《中国微生态学杂志》
CAS
CSCD
2014年第2期163-165,共3页
Chinese Journal of Microecology
基金
2012年浙江省教育厅项目(Y201223986)
2012年国家级大学生创新创业训练计划项目(201210343001)
关键词
留置管
细菌分布
生物膜
Longtime indwelling tube
Bacteria distribution
Biofilm
