摘要
建立了一种在表面活性剂OP存在下 ,用 2 - (5-溴 - 2 -吡啶偶氮 ) - 5-二乙氨基酚(5-Br-PADAP)作显色剂分光光度法同时测定铜和铁含量的新方法。用该法测定铜和铁 ,显色络合物最大吸收波长均为 558nm ;pH 3 5时 ,测得Cu - 5-Br -PADAP的ε为 9 1× 10 4L·mol- 1cm- 1,Fe- 5-Br -PADAP的ε为 7 2× 10 4 L·mol- 1·cm- 1;铜线性范围为 0~ 4 0μg/ 10mL,铁线性范围为 0~ 5 0 μg/ 10mL。此法应用于人发样品分析 ,方法简便、快速 ,结果准确、满意。
A sensitive method for simultaneous determination of copper and iron in human hair by spectrophotometry has been established.The maximum absorption of the complex compounds is at 558 nm.The molar absorption coefficients are 91×10 10L·mol -1·cm -1(Cu)and 72×10 4L·mol -1·cm -1(Fe).The linearty of tasts are 0~40μg/10mL(Cu)and 0~50(Fe).This method has applied to the analysis of human hair. The results shows the method was simple,sensitive,rapid and accurate
出处
《广东微量元素科学》
CAS
2000年第12期44-46,共3页
Trace Elements Science