摘要
研究了在ＴｒｉｔｏｎＸ－１００存在下，在ｐＨ８０的乙醇介质中，用５－Ｂｒ－ＰＡＤＡＰ作为显色剂，应用分光光度法连续测定血清中的铜、锌。本法具有较高的灵敏度，Ｃｕ２＋－５－Ｂｒ－ＰＡＤＡＰ的ε为８５０×１０４Ｌ·ｍｏｌ－１·ｃｍ－１，Ｚｎ２＋－５－Ｂｒ－ＰＡＤＡＰ的ε为９６５×１０４Ｌ·ｍｏｌ－１·ｃｍ－１。铜线性范围为０～０４５ｍｇ·Ｌ１，锌线性范围为０～０４０ｍｇ·Ｌ１。
A method for continuous determination of trace element copper and zinc in human serum by spectrophotometry with Cu2+-5-Br-PADAP in pH 80 ethanol medium and the presence of Triton X-100 has been established.The sensitivity was good. (Cu2+-5-Br-PADAP) was 850104 Lmol-1cm-1 and (Zn2+-5-Br-PADAP) was 965104 Lmol-1cm-1.The calibration curves were linear in a concentration range of 0045 mgL1 for Cu and 0040 mgL1 for Zn.
出处
《广东微量元素科学》
CAS
1999年第7期44-46,共3页
Trace Elements Science
