摘要
为了探讨低温胁迫对橡胶树耐寒新品种‘湛试327-13’和不耐寒品种‘文昌217’叶片抗氧化能力的影响,以橡胶嫁接苗为材料,模拟8℃/8℃(昼/夜)的低温胁迫,在4d内每天连续测定叶片抗氧化酶活性以及相关的生理参数,再通过激光共聚焦显微镜观察气孔保卫细胞H2O2荧光密度。结果发现:(1)低温胁迫使‘湛试327-13’和‘文昌217’叶片超氧阴离子产生速率、SOD、CAT和POD及APX活性均出现先升后降的趋势,且在整个低温胁迫中,‘湛试327-13’的各抗氧化酶活性均高于‘文昌217’;低温胁迫使‘湛试327-13’的H2O2含量在处理第1天后就达到最大值,‘文昌217’则在第3天才达到最大值。(2)两个品种叶片的H2O2含量和气孔保卫细胞的H2O2荧光强度在低温胁迫后的表现规律与上述指标基本一致。研究表明,‘湛试327-13’叶片超氧阴离子和H2O2快速积累,可能起到能促进抗氧化酶活性的提高,以及降低膜质氧化胁迫程度和TBARS含量的作用;且同时保持了较高的相对含水量和较低的相对电导率,从而提高了其对低温胁迫的耐性。
To explore the effect of chilling stress on antioxidant activities in the leaves of chilling tolerance ruble tree ＇Zhanshi 327-13＇ and chilling sensitive variety ＇Wenchang 217＇ ,we treated the seedlings of both varieties by chilling stress at 8 ℃/8 （day/night）. Antioxidant activities and the related physiological pa- rameters were determined every day from the beginning of treatment. Then, the content of H2 02 in guard cells was measured by laser confocal microscope in different time after chilling stress. The results showed that： （1）The released rate of superoxide radical anion and the activities of superoxide dismutase （SOD）, catalase （CAT）,peroxidase （POD） and APX in the leaves of ＇Zhanshi 327-13~ and ＇Wenchang 217＇ in- creased first and then decreased under chilling stress;And the activities of anti-oxidases in the leaves of ＇Zhanshi 327-13＇ were constantly higher than those in ＇Wenchang 217＇ during the whole experiment;The content of H202 maximized at the first day in the leaves of ＇Zhanshi 327-13＇ but maximized at the third day for ＇Wenchang 217＇ after chilling stress. （2）The content of H202 in the leaves was consistent with that in the guard cells. Our experimental results suggested that the quick accumulation of O2 and H2 02 in ＇Zhan- shi 327-13＇ probably played a role in molecular signaling pathways through which increased the antioxidantactivities,decreased oxidant stress and kept lower content of TBARS. Lower relative conductivity was maintained after chilling stress in the leaves of ＇Zhanshi 327-13＇ so as to increase the tolerance to chilling.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第2期311-317,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家天然橡胶产业技术体系湛江综合试验站项目(CARS-34-ZD2)
海南省自然科学基金(310077)
