摘要
目的 研究在体外培养条件下血管内皮细胞条件培养基 (ECCM)、内皮素 - 1(ET- 1)、内皮素转化酶抑制剂 phos-phoramidon对血管平滑肌细胞 (SMC)增殖的影响 ,同时研究了血管平滑肌细胞条件培养基 (SMCCM)对血管内皮细胞(EC)增殖方面的影响 .方法 EC和 SMC均来源于兔主动脉 ,在获得了 EC和 SMC的条件培养基 (CM)后 ,分别用两者以及 ET- 1进行实验 ,细胞的增殖率通过 3H掺入法进行测定 .结果 ECCM和 ET- 1可明显促进 SMC的增殖 ,并呈现剂量依赖性 .其最大效应分别为 (190± 11) % (10 0 % ECCM)和(16 6± 9) % (10 0 ng· L- 1 ET- 1) .而 phosphoramidon存在条件下的 ECCM使其分裂作用降低了 (33± 2 ) % .SMCCM抑制 EC的增殖 ,这种抑制作用并非剂量依赖性 ,其最大的抑制效应为基本水平的 (78± 3) % .结论 ECCM和 ET- 1可促进 SMC的增殖作用 .phosphoram idon可明显地抑制 ECCM对 SMC的增殖作用 .同时 SMCCM对
AIM To study the effects of vascular endothelial cell conditioned medium (ECCM), endothelin 1 (ET 1), endothelin converting enzyme inhibitor phosphoramidon on the proliferation of vascular smooth muscle cell (SMC) and vascular smooth muscle cell conditioned medium (SMCCM) on the proliferation of vascular endothelial cell (EC) in vitro . METHODS EC and SMC were isolated from rabbit aortas and cultured. The conditioned medium (CM) of EC and SMC were harvested. ECCM, ECCM conditioned in the presence of phosphoramidon, SMCCM and ET 1 were added individually to the cultures. Proliferation rate of the cells was measured with 3H thymidine incorporation method. RESULTS ECCM and ET 1 significantly increased the proliferation of SMC in a dose dependent manner with a maximal effect of (190±11)% (100%ECCM) and (166±9)% (100 ng·L -1 ET 1) respectively. ECCM conditioned in the presence of phosphoramidon reduced the mitogenic effect by (33±2)%. SMCCM elicited a dose dependent decrease of cultured EC proliferation with a maximal effect of (78±3)% over basal level. CONCLUSION ECCM and ET 1 have mitogenic effects on SMC. Phosphoramidon can significantly reduce the mitogenic effect of ECCM. SMCCM inhibits EC proliferation.
出处
《第四军医大学学报》
北大核心
2001年第1期12-15,共4页
Journal of the Fourth Military Medical University
基金
the National Natural Science Foundation of China(30070777)
关键词
血管内皮细胞
血管平滑肌细胞
细胞增殖
vascular endothelial cell
vascular smooth muscle cell
proliferation
