摘要
目的：检测细胞分裂周期蛋白2（cell division cycle associated2，CDCA2）在卵巢上皮癌组织中表达并分析其与临床病理参数的相关性，探讨CDcA2对卵巢上皮癌细胞周期和凋亡的影响。方法：选取2010-01-01-2012-12-31襄阳市第一人民医院手术切除的75例卵巢上皮癌组织标本和因良性肿瘤行手术切除的正常卵巢组织标本40例，免疫组化染色技术检测cDCA2的表达，分析不同临床病理参数下卵巢上皮癌组织中cDCA2的表达意义；应用CDCA2siRNA转染人卵巢上皮癌EFO-21细胞，蛋白质印迹法和流式细胞术检测其CDCA2表达以及细胞周期和凋亡变化。结果：CD-CA2在卵巢上皮癌组织中的免疫组化评分为20．0～190．0，中位数评分为95．0，良性卵巢评分为2．5～90．0，中位数评分为30．0，差异具有统计学意义，t=7．618，P〈0．001。CDCA2表达与卵巢上皮癌病理分级（X^2=11．265，P〈0．001）和临床分期（X^2=9．904，P=0．002）有关；siRNA干扰显著降低卵巢上皮癌EFO-21细胞中CDCA2mRNA和蛋白表达，导致Go／G1期细胞比例增加，实验组为（85．67±3．61）％，对照组为（50．50±4．37）％，t=15．188，P〈0．001；并促进细胞凋亡，实验组为（8．30±0．51）％，对照组为（2．75±0．76）％，t=14．913，P〈0．001。结论：卵巢上皮癌组织中CDCA2表达明显增高，并与恶性病理参数有关，CDCA2在卵巢上皮癌中可能通过促进细胞周期进展和抑制细胞凋亡发挥其促癌功能。
OBJECTIVE： To investigate the expression of cell division cycle associated 2 （CDCA2） and the correla- tion with clinical parameters in epithelial ovarian cancer tissues and its effects on cell cycle and apoptosis of epithelial ovar- ian cancer cells. METHODS： Seventy five epithelial ovarian cancer samples and forty normal ovarian tissues samples were selected from 2010-01-01 to 2012-12-31 in First People＇s Hospital of Xiangyang. CDCA2 expression was detected using immunohistochemistry and the clinicalpathological significance of CDCA2 in epithelial ovarian carcinoma was analyzed. Si- lence of CDCA2 with siRNA in EFO-21 ceils was confirmed by qRT-PCR and Western blot. The cell cycle profile and ap- optosis were analyzed using flow cytometry. RESULTS： The immunohistochemistry scores of CDCA2 in epithelial ovarian cancer tissues and normal ovarian tissues were 20.0-190.0 （median： 95.0） and 2.5-90.0 （median： 30.0） ,respective- ly. The difference was statistically significant （t= 7. 618, P〈0. 001）. The expression pattern of CDCA2 was associated with histological stages （X^2 = 11. 265, P〈0. 001） and clinical stage （X^2 = 9. 904, P = 0. 002）. Specific siRNA significantly downregulated the mRNA and protein level of CDCA2 in EFO-21 cells, CDCA2 knockdown increased the percentage of the G0/G1 phase,experimental group （85.67±3.61）% vs control group （50.50±4.37）% （t=15.188,P=0.001） and in- duced cell apoptosis, experimental group （8.30 ± 0.51）% vs control group （2.75 ± 0.76）% （t = 14. 913, P〈 0. 001） in EFO-21 cells. CONCLUSIONS： The expression of CDCA2 increases obviously in epithelial ovarian carcinoma and is associ- ated with poor ctinicalpathological significance. CDCA2 may act as a pro-tumor factor in epithelial ovarian caner through promoting cell cycle progression and inhibiting cell apoptosis.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2014年第6期442-446,共5页
Chinese Journal of Cancer Prevention and Treatment
