摘要
目的 :探讨脑缺血早期 (2 4h内 )大脑皮质星形胶质细胞 (Ast)的变化规律。方法 :应用胶质原纤维性酸性蛋白免疫组织化学ABC技术。结果 :缺血 1 5min和 3 0min组 ,缺血中心区大脑皮质胶质原纤维性酸性蛋白阳性细胞较均匀地分布于II～VI层 ,细胞数量明显多于非缺血区 ;缺血 1h和 2h组胶质原纤维性酸性蛋白阳性细胞数量进一步增加 ,胞质染色加深 ,并集中出现于III、IV层 ;缺血 3h组胶质原纤维性酸性蛋白阳性细胞进一步增大呈气球样 ,突起增长变粗 ,突起内出现水肿泡 ;缺血 6h组胶质原纤维性酸性蛋白细胞固缩 ,边界不清 ;1 2h和 2 4h组缺血中心区胶质原纤维性酸性蛋白细胞消失。同时观察到缺血 3h和 6h组缺血边缘区胶质原纤维性酸性蛋白细胞数量增多 ,直径增大 ,并可见到细胞分裂现象。结论 :星形胶质细胞对脑缺血早期神经元损伤具有较强的保护作用。
Objective: To clarifiy the changes of the astrocytes (Ast) during early stage (within 24 hours) of focal cerebral ischemia. Methods: Using ABC immunohistochemical technique for GFAP. Results: GFAP-positive cells were evenly distributed between layers of II～VI of ischemia foci in the cerebral cortex in 15 min and 30 min ischemia groups, and the numbers of which were more than those in normal cerebral regions. In 1 hour and 2 hours groups, the numbers of GFAP-positive cells increased, staining of their cytoplasms were darker, and concentrated at layers III and IV. In 3 hours group, the GFAP-positive cells became a balloon-like in shape, and their processes enlarged and lengthened farther, and some edema blebs emerged. As for 6 hour group, the GFAP-positive cells were shrunken with obscure contours. In 12 hours and 24 hours groups, the GFAP-positive cells disappeared. At ischemia border zone, the GFAP-positive cells increased in number and cell division appeared in 3 hours and 6 hours groups. Conclusion: Astrocytes play a protective role for damaged neurons at early stage during cerebral ischemia.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2001年第1期20-23,共4页
Chinese Journal of Anatomy
基金
河北省自然科学基金 (No :3 95 3 5 7)
