摘要
目的探讨不同睡眠剥夺方式对大鼠学习记忆能力的影响及其可能机制。方法将24只大鼠用随机数字法分为空白组(不做任何处理140h)、对照组(连续睡眠剥夺120h)、实验1组(睡眠剥夺共计120h,分3次进行,每次40h;间歇2次,每次10h,共间歇20h)和实验2组(睡眠剥夺共计120h,分6次进行,每次20h;间歇5次,每次4h,共间歇20h)。睡眠剥夺后用旷场反应箱测试大鼠的行为,用Morris水迷宫法测试大鼠学习记忆能力,用试剂盒检测大鼠大脑皮层、海马和血浆中超氧化物歧化酶(SOD)和丙二醛(MDA)含量以及大脑皮层和海马中乙酰胆碱酯酶(TChE)含量。结果旷场反应测试大鼠兴奋性和探索性行为,实验组明显强于对照组,且实验2组强于实验1组(P<0.01);水迷宫测试大鼠学习记忆能力,实验组明显强于对照组,且实验2组强于实验1组(P<0.01);实验组大鼠大脑皮层、海马和血浆中SOD含量明显高于对照组,且实验2组高于实验1组(P<0.01);实验组大鼠大脑皮层、海马和血浆中MDA含量明显低于对照组(P<0.01),且实验2组低于实验1组;实验组大鼠大脑皮层和海马TChE含量明显低于对照组,且实验2组低于实验1组(P<0.01)。结论不同的间断性睡眠剥夺方式对大鼠学习记忆能力有不同的影响,一次性睡眠剥夺时间越长、间歇次数越少,学习记忆能力越差。这种作用可能与睡眠剥夺抑制了大脑皮层、海马和血浆中SOD活性,提高了MDA含量及TChE活性有关。
Objective To explore the effects of different methods of sleep deprivation on rat learning and memory and the possible mechanisms. Methods Twenty-four rats were randomly divided into blank group （untreated for 140 h）, control group （120 h continuous sleep deprivation）, experimental group 1 （120 h of sleep deprivation in total, for three times, each time lasting 40 h; intermittent twice, each lasting 10 h, intermittent 20 h in total）, and experimental groups 2 （120 h of sleep deprivation in total, for six times, each time lasting 20 h; intermittent five times, each lasting 4 h, intermittent 20 h in total）. After intermittent sleep deprivation, the rats＇ behavior was measured by open field test; their learning and memory abilities were tested with Morris water maze. Then superoxide dismutase （SOD）, malondialdehyde （MDA） and total cholinesterase （TChE） contents in the cerebral cortex, hippocampus and blood plasma of the rats were measured. Results The results of both open field test and water maze test showed that excitatory and exploratory behavior as well as learning and memory abilities were significantly better in the experimental groups than in the control group, with experimental group 2 being better than experimental group I （both P〈0.01）. The content of SOD in the cerebral cortex, hippocampus and plasma was significantly higher in the experimental groups than in the control group （P〈0.01）, experimental group 2 being higher than experimental group 1. The content of MDA in the cerebral cortex, hippocampus and plasma was significantly lower in the experimental groups than in the control group （P〈0.01）. TChE content in the cerebral cortex and hippocampus was significantly lower in the experimental groups than in the control group （P〈0.01）,experimental group 2 being lower than experimental group 1. Conclusion Different methods of intermittent sleep deprivation had significant effects on rat learning and memory. Moreover, the longer onc-time sleep deprivation and fewer interwlls, the poorer learning and memory abilities are. This effect may be associated with the inhibition of SOD activities in the cortex, hippocampus and plasma by sleep deprivation, and increases of MDA content and TChE activity.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2014年第2期213-217,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省科学技术研究发展计划项目(No.2011K15-06-10)~~
关键词
睡眠剥夺
学习记忆
行为
超氧化物歧化酶
丙二醛
乙酰胆碱酯酶
sleep deprivation
learning and memory
behavior
superoxide dismutase （SOD）
malondialdchydc（MDA）
total cholincsterasc （TChE）
