摘要
采用Ｍｏｒｉｓ水迷宫法和ＮＢＴ法分别检测了褪黑激素对Ｄ半乳糖处理小鼠的空间学习记忆能力和脑组织超氧物歧化酶活力的影响。结果表明，Ｄ半乳糖对小鼠的逃避潜伏期无显著影响，但降低小鼠的穿环系数，还促使小鼠的脑组织超氧物歧化酶活力明显升高；褪黑激素可提高小鼠的空间学习记忆能力，降低Ｄ半乳糖所引起的超氧物歧化酶活力升高。
Forty NIH mice were divided into 4 groups and received daily i p. injection of 0 9% NaCl (control group), D galactose(250 mg/kg d), melatonin(2 5 mg/kg d) and melatonin(2 5 mg/kg d)+D galactose(250 mg/kg d) respectively. All animals were maintained at a light dark cycle of 12 L∶12 D. Morris water maze was used to test the escaping latencies and the anulus crossing percentage of animals during the 37th 42nd days after treatment. The results wre as follows: 1) the escaping latencies: control group, 60 2±1 43 s; D galatose group, 59 4±2 69 s; melatonin+D galactose group, 43 4±2 93 s; 2)the anulus crossing percentage:control group, 37 4±8 5%; D galactose grouup, 28 5±8 7%; melatonin+D galactose group, 49 7±13 4%. On the 43rd day, all mice were killed and their brain tissues were removed and separated into two parts:hypothalamus and non hypothalamus. The SOD activities of the brain tissues were determined. The result showed that the SOD activities wre higher in hypothalamus than in non hypothalamus. The SOD activities in hypothalamus were D galactose group(299 01±8 87 U/100mg wet wt.); melatonin +D galactose group(284 7±23 63); control group(269 83±17 16); melatonin group(258 39±16 28) respectively. These results suggest that melatonin may improve the learning ability of mice and decrease the SOD activities in hypothalamus. The latter may result from a physiological compensation effect.
出处
《动物学报》
SCIE
CAS
CSCD
1998年第1期61-66,共6页
ACTA ZOOLOGICA SINICA
关键词
褪黑激素
D-半乳糖
脑组织
学习
记忆
Mice, Melatonin, D galactose, Brain Tissues,Learning and Memory, SOD
