摘要
目的观察睡眠剥夺对大鼠脑组织γ-氨基丁酸(GABA)、谷氨酸(GLU)含量的影响。方法用小平台水环境法对大鼠进行连续96 h快速动眼相睡眠剥夺,以正常饲养组及大平台组为对照,高效液相色谱法测定睡眠剥夺后大鼠额叶皮质、脑干、下丘脑等脑区GABA、GLU含量的变化。结果睡眠剥夺后大鼠各脑区脑组织GABA含量、GABA/GLU值均较对照组明显增高(P<0.01),而GLU含量在脑干、下丘脑两脑区亦较对照组明显增高(P<0.05)。结论睡眠剥夺可伴有大鼠脑组织氨基酸类神经递质含量及比例的变化。
Objective To investigate the effects of sleep deprivation on the contents of γ-amino-butyric acid (GABA) and glutamate (GLU) in rat brain. Methods Thirty rats were assigned into sleep-deprivation (SD, n=10) group (subjected to treatment on small platforms 6.5 cm of diameter), tank control (TC, n=10) group (treated on large platforms 14 cm of diameter) and control group kept in cages (CC, n=10). Rats in SD group were deprived of rapid eye movement sleep for 96 h by 'flower pot' technique. High-performance liquid chromatography was used to determine the GABA and GLU contents in the 3 brain regions of the rats: the frontal cortex, hypothalamus and brain stem. Results The rats in SD group showed an increase in the contents of GABA and lowered ratio of GLU/GABA in all the 3 brain regions (P<0.01) compared with the rats in CC group; GLU contents were also increased in the brain stem and hypothalamus of the rats in SD group (P<0.05). No significant changes were found in TC group. Conclusion Rapid eye movement sleep deprivation induces significant changes in the contents and proportion of amino-acid neurotransmitter in rat brain.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第10期888-890,共3页
Journal of First Military Medical University
基金
全军"九五"医学科研规划项目(96M061)
关键词
睡眠剥夺
脑化学
氨基酸神经递质类
GABA
谷氨酸
sleep deprivation
brain chemistry
amino acid neurotransmitters
γ-amino-butyric acid
glutamic acid
