摘要
利用新一代高通量测序手段——转录组测序(RNA-Seq)技术研究在300 mmol·L-1 NaCl胁迫下两个甜瓜(Cucumis melo L.)品种‘玉露’和‘冰雪脆’的转录因子基因表达变化。这两个甜瓜品种在叶绿素荧光参数上的差异表明其在盐胁迫下有不同的生理反应。转录组测序结果表明,盐胁迫与对照相比,‘玉露’共有属于19个转录因子家族的56个转录因子基因表达发生变化(在转录水平,属于7个转录因子家族的22个转录因子上调表达,属于14个转录因子家族的34个转录因子下调表达)。‘冰雪脆’有属于20个转录因子家族的47个转录因子基因表达发生变化(在转录水平上,属于5个转录因子家族的17个转录因子上调表达,属于17个转录因子家族的30个转录因子下调表达)。盐胁迫下,两个甜瓜品种差异表达的转录因子既表现特异性,也存在部分重叠。‘玉露’有29个转录因子特异响应,‘冰雪脆’有20个特异响应。盐胁迫响应重叠的转录因子有27个,其中9个上调表达,18个下调表达。采用实时荧光定量PCR对几个转录因子进行了盐胁迫下的表达检测,其趋势与转录组分析结果基本一致。
The expression changes of transcription factor genes in two melon（Cucumis melo L.） cultivars（‘Yvlu’ and ‘Bingxuecui’） were analyzed under salt stress by the next-generation high-throughput sequencing technology—RNA-Seq.The differences in the chlorophyll fluorescence parameters between these two cultivars indicated significant difference in their salt tolerance.The results showed that 56 differential expressed transcription factor genes belonged to 19 transcription factors families of ‘YL’（at the transcription level,22 upregulated genes were belonged to 7 transcription factors families and 34 down-regulated were belonged to 14 transcription factors families） and 47 transcription factor genes belonged to 20 transcription factors families of ‘BXC’（at the transcription level,17 up-regulated genes were belonged to 5 transcription factors families and 30 down-regulated genes were belonged to 17 transcription factors families） under salt stress.The expression of transcription factors genes was affected by salt-stress in melon,and there were genes of cultivar specialresponse as well as there were genes overlap between ‘YL’ and ‘BXC’.29 transcription factors from ‘YL’ and 20 from ‘BXC’ were cultivar-special in response to salt stress respectively.There were 27 transcription factor genes exhibited the same current in response to salt stress in both ‘YL’ and ‘BXC’,with 9 up-regulated and 18 down-regulated at the transcription level,respectively.Finally,the results of transcriptome analyses were verified by real-time quantitative PCR.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第2期150-158,共9页
Plant Physiology Journal
基金
国家自然科学基金(31372079)
上海市自然科学基金(13ZR1422400)
上海市园艺学重点学科建设项目
关键词
甜瓜
盐胁迫
高通量测序
转录因子
转录组分析
melon（Cucumis melo）
salt stress
high-throughput sequencing
transcription factors
transcriptome analysis
