摘要
目的探讨 ＣＳ２对下丘脑－垂体－卵巢轴平衡调节功能的影响机制。方法对 ＳＤ大鼠给予不同剂量 ＣＳ２（每天４００ ｍｇ／ｋｇ、１００ ｍｇ／ｋｇ）染毒不同时间（１４ ｄ、２８ ｄ）后，测定大风动情期血清中激素ＦＳＨ、ＬＨ、Ｐ、Ｅ２含量变化及动情间期给予ＧｎＲＨ刺激试验。结果随着染毒剂量的增大或染毒时间的延长，大鼠均不同程度地出现动情周期延长，甚至出现动情期消失，ＣＳ２染毒大鼠卵巢重／体重比值与对照组比较，显著增高且具有显著差异（Ｐ＜０．０５）。各染毒组（每天４００ｍｇ／ｋｇ、１００ ｍｇ／ｋｇ染毒 １４ ｄ和 １００ ｍｇ／ｋｇ染毒 ２８ ｄ）与对照组比较，血清 ＬＨ水平显著增高，且有显著性差异（Ｐ＜ ０．０５）。除高剂量组大鼠在注射ＧｎＲＨ６０ｍｉｎ后血清Ｐ的含量显著高于对照组及低剂量组，且均有显著性差异（Ｐ＜０．０５）外，各剂量组的大鼠血清的各激素水平均未免显著性差异（Ｐ＞０．０５）。结论在本实验条件下，ＣＳ２对雌性性腺功能具有损害作用，ＧｎＲＨ刺激试验结果提示垂体储备功能尚未降低，但不能排除是由于大鼠对ＧｎＲＨ的超度反应所致。
Objective To explore the toxic mechanism of carbon disulfide (CS2) in term of adjusting and balancing function of the hypothalamic-pituitary- ovary axis. Methods SD rats were given CS2 with dose of 400 mg/ kg and 100 mg/ kg for 14 days and 28 days respectively and the changes of serum follicle- stimulating- hormone (FSH ), luteinizing hormone (LH), Progesterone (P) and estrogen (E2) in rat estrus were measured. In addition, rat in estrus was given gonadotropic-release hormone (GnRH) to stimulate pituitary secretion. Results As exposure dose increasing and exposure time prolonging, it was showed that the time of estrus cycle in rats was delayed to certain extent and even rats' estrus seemed to be disappeared. The ratio of ovary to body weight in CS2 group was increased significantly with comparison to that of control group (P < 0. 05). LH was significantly increased in those two treated groups than that in control group (P < 0. 05). Except that serum levle of P of high dose group was significantly increased 60 minutes after injection of GnRH than that of low dose group and control group (P < 0. 05), there was no difference of serum hormone level among each group (P > 0. 05). Conclusion It concluded that CS2 might have adverse effect on female-gonad and indicated that the reservating function of pituitary in rats was not reduced in GnRH-stimulating-test, however, the possibility of GnRH super-response in rat was unable to be excluded.
出处
《中国职业医学》
CAS
北大核心
2001年第1期12-14,共3页
China Occupational Medicine
