摘要
主要研究了几种单、双子叶植物种子在人工环境条件下的发芽率及对几种常用溶剂的反应,并选用了几种合适的种子以及小球藻(Chlorella vulgaris)、浮萍(Lemnaceae)对9种除草剂进行了除草活性试验。结果表明,不同备选靶标在室内人工环境适应性及对除草剂活性反应均不同;综合比较各备选靶标的发芽率、培养温度、对常用溶剂及除草剂的敏感性等因素,确定了几个可用于除草剂组合筛选的靶标。以组合靶标方式建立的除草剂筛选流程提供的是一个多重筛选模型,可减少对低活性先导化合物的漏筛。近3年来对30 000多个微生物源提取物的筛选结果表明,运用组合靶标是一种稳定、高效的除草活性筛选模式。
20 different seeds were germinated in different temperature.Effects of different solvents on the targets of herbicide were studied.The herbicidal activity of 9 herbicides against 5 different targets was investigated.The results showed that the combination of target was stable and highly efficient,simple,sensitive for screening herbicide.The screening method based on the combination of target can offer the multiple sieves to ensure that leading compounds with low activity will not be lost.More than 30 000 extracts from microorganism were screened with the combination of target in the past three years.The results showed that the method is stable and efficient.
出处
《湖北农业科学》
北大核心
2013年第23期5757-5759,5810,共4页
Hubei Agricultural Sciences
基金
公益性行业(农业)专项(200903052)
关键词
组合靶标
除草活性
微生物源提取物
模式靶标
combination of target
herbicidal activity
extracts from microorganism
model target
